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Skin Tissue Engineering: Methods and Protocols Second Edition 2025 [Hardback]

  • Formāts: Hardback, 358 pages, height x width: 254x178 mm, 96 Illustrations, color; 10 Illustrations, black and white; XVI, 358 p. 106 illus., 96 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2922
  • Izdošanas datums: 11-Apr-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071645099
  • ISBN-13: 9781071645093
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  • Hardback
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  • Formāts: Hardback, 358 pages, height x width: 254x178 mm, 96 Illustrations, color; 10 Illustrations, black and white; XVI, 358 p. 106 illus., 96 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2922
  • Izdošanas datums: 11-Apr-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071645099
  • ISBN-13: 9781071645093
Citas grāmatas par šo tēmu:

This second volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. Chapters describe the isolation, culture of diverse skin cells, skin models, and managing these cells within co-culture systems. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Skin Tissue Engineering: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.

 


Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell
Co-culture.- Isolation and Culture of Epidermal Melanocytes.- Initiating
Separate Cultures of Keratinocytes and Melanocytes from a Single Biopsy.-
Long-term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-beta
Signaling Inhibitor.- Isolation and Culture of Hair Follicle Dermal Sheath
Mesenchymal Stromal Cells.- Isolation and Culture of Human Dermal
Fibroblasts.- Isolation and Culture of Human Dermal Microvascular Endothelial
Cells Jennifer Bourland.- Isolation of stromal vascular fraction by
fractionation of adipose tissue.- Engineering a multilayered skin equivalent:
the importance of endogenous extracellular matrix and model flexibility for
nuanced and accurate skin studies.- Engineering a Multilayered Skin
Substitute with Keratinocytes, Fibroblasts, Adipose Derived Stem Cells and
Adipocytes.- Isolation and Culture of Epidermolysis Bullosa Cells and
Organotypic Skin Models.- Engineering a pigmented skin equivalent that is
responsive to external stimuli.- Bioprinting a tri-layered skin analogue.-
Transdifferentiation of human dermis derived fibroblasts into iNeurons and
cultivation in a collagen type 1 hydroge.- Deep proteome profiling of primary
skin fibroblasts.- Characterization of human skin derived cells by Raman
micro-spectroscopy.- Standard Preparation Protocol of Human Skin and Skin
Substitute Samples for Transmission Electron Microscopy.- Methods for
assessing scaffold vascularization with human endothelial cells.-
Histological and histochemical methods for the evaluation of tissue
engineered skin substitutes.- Pig Model to Test Tissue engineered Skin.-
Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig
Model.- Ex vivo human skin model to study immediate and early local responses
to external stimuli.- A Method to Evaluate Skin and Skin Substitute
Mechanical Properties Using a Suction Device.- GMP compliant production of
therapeutic components of autologous adipose tissue.- Quality controls of
autologous skin products according to European GMP guidelines.- Standardized
outcome measures for the clinical application of tissue engineered products.