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E-grāmata: Sphingolipid Metabolism and Cell Signaling, Part B

Volume editor (Medical University of South Carolina, Charleston, U.S.A.), Editor-in-chief (California Institute of Technology, Division of Biology, Pasadena, U.S.A.), Editor-in-chief (The Salk Institute, La Jolla, CA, USA), Volume editor (Emory University, Atlanta, Georgia, U.S.A.)
  • Formāts: PDF+DRM
  • Sērija : Methods in Enzymology
  • Izdošanas datums: 25-Oct-2000
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780080496696
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Sphingolipid Metabolism and Cell Signaling, Part BPalielināt
  • Formāts: PDF+DRM
  • Sērija : Methods in Enzymology
  • Izdošanas datums: 25-Oct-2000
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780080496696
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This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Recenzijas

Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be Colowick and Kaplan's multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Contributors to Volume 312 xi Preface xvii Volumes in Series xix Section I. Methods for Analyzing Sphingolipids Analysis of Sphingoid Bases and Sphingoid Base 1-Phosphates by High-Performance Liquid Chromatography 3(6) Alfred H. Merrill, Jr. Thomas B. Caligan Elaine Wang Katherine Peters Joyce Ou Enzymatic Method for Measurement of Sphingosine 1-Phosphate 9(7) Lisa Edsall Lewis Vann Sheldon Milstien Sarah Spiegel Ceramide Mass Analysis by Normal-Phase High-Performance Liquid Chromatography 16(6) Annemiek D. Tepper Wim J. van Blitterswijk Quantitative Determination of Ceramide Using Diglyceride Kinase 22(10) David K. Perry Alicja E. Bielawska Yusuf A. Hannun Analysis of Sphingomyelin, Glucosylceramide, Ceramide, Sphingosine and Sphingosine 1-Phosphate by Tandem Mass Spectrometry 32(13) M. Cameron Sullards Analyses of Glycosphingolipids by High-Performance Liquid Chromatography 45(19) Johannes Muthing Sphingolipid Extraction and Analysis by Thin-Layer Chromatography 64(16) Gerhild Van Echten-Deckert Extraction and Analysis of Multiple Sphingolipids from a Single Sample 80(21) Tamotsu Taketomi Eiko Sugiyama Purification of Sphingolipid Classes by Solid-Phase Extraction with Aminopropyl and Weak Cation Exchanger Cartridges 101(14) Jacques Bodennec Gerard Brichon Georges Zwingelstein Jacques Portoukalian Ganglioside Analysis by High-Performance Thin-Layer Chromatography 115(20) Toshio Ariga Robert K. Yu Purification and Analysis of Gangliosides 135(10) Stephan Ladisch Ruixiang Li Thin-Layer Chromatography Blotting Using Polyvinylidene Difluoride Membrane (Far-Eastern Blotting) and Its Applications 145(12) Dai Ishikawa Takao Taki Thin-Layer Chromatography Immunostaining 157(3) Dai Ishikawa Takao Taki Monoclonal Anti-Glycosphingolipid Antibodies 160(19) Reiji Kannagi Immunolocalization of Gangliosides by Light Microscopy Using Anti-Ganglioside Antibodies 179(8) Andreas Schwarz Anthony H. Futerman Cloud-Point Extraction of Gangliosides Using Nonionic Detergent C14EO6 187(9) George C. Terstappen Anthony H. Futerman Andreas Schwarz Analyses of Glycosphingolipids Using Clam, Mercenaria mercenaria, Ceramide Glycanase 196(9) Sara Dastgheib Shib S. Basu Zhixiong Li Manju Basu Subhasu Basu Quantitative Analyses of Binding Affinity and Specificity for Glycolipid Receptors by Surface Plasmon Resonance 205(12) C. Roger MacKenzie Tomoko Hirama Use of Circular Dichroism for Assigning Stereochemistry of Sphingosine and Other Long-Chain Bases 217(11) Akira Kawamura Koji Nakanishi Nina Berova Infrared Determination of Conformational Order and Phase Behavior in Ceramides and Stratum Corneum Models 228(19) Richard Mendelsohn David J. Moore Use of Nuclear Magnetic Resonance Spectroscopy in Evaluation of Ganglioside Structure, Conformation, and Dynamics 247(25) Domenico Acquotti Sandro Sonnino Fluorescence Quenching Assay of Sphingolipid/Phospholipid Phase Separation in Model Membranes 272(21) Erwin London Deborah A. Brown Xiaolian Xu Section II. Methods for Analyzing Aspects of Sphingolipid Metabolism in Intact Cells Synthesis of Fluorescent Substrates and Their Application to Study of Sphingolipid Metabolism in Vitro and in Intact Cells 293(11) Arie Dagan Vered Agmon Shimon Gatt Tama Dinur Selection of Mammalian Cell Mutants in Sphingolipid Biosynthesis 304(13) Kentaro Hanada Masahiro Nishijima Selection of Yeast Mutants in Sphingolipid Metabolism 317(13) Teresa M. Dunn Ken Gable Erin Monaghan Dagmar Bacikova Fluorescence-Based Selection of Gene-Corrected Hematopoietic Stem and Progenitor Cells Based on Acid Sphingomyelinase Expression 330(9) Edward H. Schuchman Shai Erlich Silvia R. P. Miranda Tama Dinur Arie Dagan Shimon Gatt Mammalian Ganglioside Sialidases: Preparation and Activity Assays 339(22) Erhard Bieberich Sean S. Liour Robert K. Yu Section III. Sphingolipid-Protein Interactions and Cellular Targets Effects of Sphingosine and Other Sphingolipids on Protein Kinase C 361(12) E. R. Smith Alfred H. Merrill, Jr. Lina M. Obeid Yusuf A. Hannun Kinetic Analysis of Sphingoid Base Inhibition of Yeast Phosphatidate Phosphatase 373(8) Wen-I. Wu George M. Carman Assays of Sphingosine-Dependent Kinase for 14-3-3 Protein 381(6) Tamar Megidish Akikazu Hamaguchi Kazuhisa Iwabuchi Sen-Itiroh Hakomori Synthesis and Use of Caged Sphingolipids 387(14) Roderick H. Scott Jamie Pollock Ahmet Ayar Nicola M. Thatcher Uri Zehavi Binding of Sphingosine 1-Phosphate to Cell Surface Receptors 401(6) James R. Van Brocklyn Sarah Spiegel Use of Short-Chain Ceramides 407(13) Chiara Luberto Yusuf A. Hannun Analysis of Ceramide-Activated Protein Phosphatases 420(9) Charles E. Chalfant Katsuya Kishikawa Alicja Bielawska Yusuf A. Hannun Use of Affinity Chromatography and TID-Ceramide Photoaffinity Labeling for Detection of Ceramide-Binding Proteins 429(9) Stefan Schutze Marc Wickel Michael Heinrich Supandi Winoto-Morbach Thomas Weber Josef Brunner Martin Kronke Lectin-Mediated Cell Adhesion to Immobilized Glycosphingolipids 438(9) Brian E. Collins Lynda J.-S. Yang Ronald L. Schnaar Analysis of Glycolipid-Dependent Cell Adhesion Based on Carbohydrate-Carbohydrate Interaction 447(12) Kazuko Handa Naoya Kojima Sen-Itiroh Hakomori Analysis of Interactions between Glycosphingolipids and Microbial Toxins 459(14) Clifford A. Lingwood Beth Boyd Anita Nutikka Oxidation of Aglycone of Glycosphingolipids: Serine and Ceramide Acid Precursors for Soluble Glycoconjugates 473(15) Murugesapillai Mylvaganam Clifford A. Lingwood Separation of Glycosphingolipid-Enriched Microdomains from Caveolae Characterized by Presence of Caveolin 488(7) Kazuhisa Iwabuchi Kazuko Handa Sen-Itiroh Hakomori Reconstitution of Sphingolipid-Cholesterol Plasma Membrane Microdomains for Studies of Virus-Glycolipid Interactions 495(11) Djilai Hammache Gerard Pieroni Marc Maresca Serge Ivaldi Nouara Yahi Jacques Fantini Analysis of Ceramides Present in Glycosylphosphatidylinositol Anchored Proteins of Saccharomyces cerevisiae 506(9) Isabelle Guillas Martine Pfefferli Andreas Conzelmann Preparation of Functionalized Lipid Tubules for Electron Crystallography of Macromolecules 515(8) Elizabeth M. Wilson-Kubalek Section IV. Sphingolipid Transport and Trafficking Applications of BIODIPY-Sphingolipid Analogs to Study Lipid Traffic and Metabolism in Cells 523(11) Richard E. Pagano Rikio Watanabe Christine Wheatley Michel Dominguez Using Biotinylated Gangliosides to Study Their Distribution and Traffic in Cells by Immunoelectron Microscopy 534(28) Gunter Schwarzmann Alexander von Coburg Wiebke Mobius Assays for Transmembrane Movement of Sphingolipids 562(21) Dan J. Sillence Rene J. Raggers Gerrit van Meer Section V. Other Methods Compilation of Methods Published in Previous Volumes of Methods in Enzymology 583(10) Alfred H. Merrill, Jr. Author Index 593(38) Subject Index 631
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