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E-grāmata: Stem Cells and Aging: Methods and Protocols

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  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 976
  • Izdošanas datums: 12-Feb-2013
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781627033176
  • Formāts - PDF+DRM
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  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 976
  • Izdošanas datums: 12-Feb-2013
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781627033176

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Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome. Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for understanding these age-related stem cell changes at a basic biology level and at the level of their impacts for regenerative medicine. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy to use, Stem Cells and Aging: Methods and Protocols serves as an ideal reference to guide investigators toward further valuable answers to the problems of our aging population.

This Methods in Molecular Biology™ book presents protocols critical for exploring the biology of stem cell aging, which is vital for understanding age-related stem cell changes at a basic biology level and at the level of their impact on regenerative medicine.
Preface v
Contributors ix
1 Comprehensive Hematopoietic Stem Cell Isolation Methods
1(16)
Kyle Rector
Yi Liu
Gary Van Zant
2 Serial Transplantation of Bone Marrow to Test Self-renewal Capacity of Hematopoietic Stem Cells In Vivo
17(8)
Charusheila Ramkumar
Rachel M. Gerstein
Hong Zhang
3 Mouse Hematopoietic Stem Cell Transplantation
25(12)
Hui Cheng
Paulina H. Liang
Tao Cheng
4 Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells
37(16)
Mladen I. Tovchev
Mariana D. Dabeva
Michael Oertel
5 Isolation of Muscle-Derived Stem/Progenitor Cells Based on Adhesion Characteristics to Collagen-Coated Surfaces
53(14)
Mitra Lavasani
Aiping Lu
Seth D. Thompson
Paul D. Robbins
Johnny Huard
Laura J. Niedernhofer
6 Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and Cytochemical Analyses at Light and Electron Microscopy
67(14)
Manuela Malatesta
Marzia Giagnacovo
Rosanna Cardani
Giovanni Meola
Carlo Pellicciari
7 Cardiac Stem Cell Senescence
81(18)
Daniela Cesselli
Federica D'Aurizio
Patrizia Marcon
Natascha Bergamin
Carlo Alberto Beltrami
Antonio Paolo Beltrami
8 Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions
99(12)
Sebastian Klepsch
Angelika Jamnig
Daniela Trimmel
Magdalena Schimke
Werner Kapferer
Regina Brunauer
Sarvpreet Singh
Stephan Reitinger
Gunter Lepperdinger
9 Methods for Assessing Effects of Wnt/β-Catenin Signaling in Senescence of Mesenchymal Stem Cells
111(20)
Hai-jie Wang
Yu-zhen Tan
10 Intra-femoral Injection of Human Mesenchymal Stem Cells
131(12)
Sindhu T. Mohanty
Ilaria Bellantuono
11 Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency
143(12)
Anne Schellenberg
Hatim Hemeda
Wolfgang Wagner
12 Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging
155(12)
Seunghee Lee
Ji-Won Jung
Kyung-Sun Kang
13 Quantitative Fluorescence In Situ Hybridization on Paraffin Embedded Tissue
167(8)
Mario Ricciardi
Mauro Krampera
Marco Chilosi
Index 175