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E-grāmata: Teratogenicity Testing: Methods and Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2753
  • Izdošanas datums: 29-Jan-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071636251
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Teratogenicity Testing: Methods and ProtocolsPalielināt
  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2753
  • Izdošanas datums: 29-Jan-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071636251
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This fully updated volume collects laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. Beginning with a section detailing basic principles of teratogenicity, the book continues with application of in vitro models for teratogenic screening to predict toxicity in embryonic animals and humans, as well as a variety of well-established in vivo animal model tests, which offer solutions for evaluating the efficacy and the teratogenicity of compounds in preclinical trials before human safety testing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Teratogenicity Testing: Methods and Protocols, Second Edition serves as an ideal guide to tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.
Updating an Overview of Teratology.- Guidelines on Development Toxicity
Tests: Brief Insights.- Development Features on the Selection of Animal
Models for Teratogenic Testing.- Virus as Teratogenic Agents.- The Future of
the Teratogenicity Testing.- The First Steps on AOPs' Concepts, Development,
and Applications in Teratology.- Machine Learning to Predict Teratogenicity:
Theory and Practice.- Human Pluripotent Stem Cell-Based Assays to Predict
Developmental Toxicity.- Developmental Toxicity Using the Rat Whole Embryo
Culture.- A Modified Murine Embryonic Stem Cell Test for Evaluating the
Teratogenic Effects of Drugs.- Teratogenic Effects of Drugs on Primary
Lymphocytes Assessed by Flow Cytometry.- Assessment of the Teratogenic Effect
of Drugs on the Chicken Embryo.- Daphnia magna as a Model Organism to Predict
the Teratogenic Effect of Different Compounds.- Caenorhabditis elegans as an
In Vivo Model Organism to Elucidate Teratogenic Effects.- Enduring
Ethanol-Induced Behavioral Alterations in Caenorhabditis elegans after
Developmental Lead Exposure.- Exploration of Teratogenic and Genotoxic
Effects on Model Organism Drosophila melanogaster.- Ecotoxicological
Assessment of Seaweed-Based Crop Biostimulant on Earthworm Eudrilus eugeniae
Kinb.- Evaluation of the Ecotoxicology of Seaweed-Based Biopesticide Used in
Combat of the Polyphagous Pest Using Eudrilus eugeniae Kinb.- Biochemical
Studies to Understand Teratogenicity and Lethality Outcomes in
Modified-FETAX.- Bioinformatics Methods for Transcriptome Analysis on
Teratogenesis Testing.- Angiogenesis Assay for Live and Fixed Zebrafish
Larvae.- High-Resolution Respirometry for the Assessment of Teratogenic
Chemicals.- A Stereological Approach to Quantify Immunohistochemical Staining
in Zebrafish Larvae.- Whole-Mount Immunohistochemical and Immunofluorescence
Assays in Zebrafish Embryos.- Assessment of Developmental Neurotoxicity Using
Semi-Automatic Behavior Analysis System for Zebrafish.- Behavioral Profiling
of Zebrafish (Danio rerio) Larvae: Activity, Anxiety, Avoidance, and Startle
Response.- Alcian Blue Staining for Chondrocranium Development in Zebrafish.-
Protocol of Geometric Morphometrics for Teratogenicity Testing.- Biochemical
Markers for Liver Injury in Zebrafish Larvae.- Cortisol Quantification for
Assessing Stress-Induced Changes in Zebrafish Larvae.- Metabolomic
Fingerprint Assay in Zebrafish Embryos.- Alkaline Comet Assay to Assess
Genotoxicity in Zebrafish Larvae.- A Chromogenic Quantification of Protein
Expression in Zebrafish Larvae.- Confocal Microscopy Technique in
Teratogenicity Testing Using Zebrafish (Danio rerio) Embryos as Model.-
Whole-Mount RNA In Situ Hybridization of Zebrafish Embryos.- Quantitative
Real-Time PCR Method to Evaluate Gene Expression in Zebrafish Embryos.-
Microarray Analysis to Determine Gene Expression Changes in Zebrafish
Embryos.- Cell-Free DNA and Next-GenerationSequencing for Prenatal Diagnosis.
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