Textbook of Assisted Reproductive Techniques has become a classic comprehensive reference for the whole team at the IVF clinic. The fourth edition comes more conveniently as a set of two separate volumes, one for laboratory aspects and the other for clinical applications.
The text has been extensively revised, with the addition of several important new contributions on laboratory aspects including developing techniques such as PICSI, IMSI, and time-lapse imaging. The second volume focuses on clinical applications and includes new chapters on lifestyle factors, tailored ovarian stimulation, frozen-thawed embryo transfer, viral disease, and religious perspectives. As before, methods, protocols, and techniques of choice are presented by eminent international experts.
The two volume set includes:
Volume One - Laboratory Perspectives Volume Two - Clinical Perspectives
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| The beginnings of human in vitro fertilization |
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| Robert Edwards: The path to IVF |
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I Establishing and Maintaining an IVF Laboratory |
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1 Setting up an ART laboratory |
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1 | (8) |
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2 Quality control: Maintaining stability in the laboratory |
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9 | (22) |
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3 The ART laboratory: Current standards |
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31 | (17) |
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II Gamete Collection, Preparation and Selection |
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48 | (13) |
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5 Sperm preparation techniques |
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61 | (14) |
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6 Sperm chromatin assessment |
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75 | (21) |
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7 Oocyte retrieval and selection |
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96 | (18) |
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8 Preparation and evaluation of oocytes for ICSI |
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114 | (8) |
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9 Hyaluronic acid binding-mediated sperm selection for ICSI |
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122 | (13) |
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10 Intracytoplasmic morphologically selected sperm injection |
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135 | (16) |
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11 Use of in vitro maturation in a clinical setting: Patient populations and outcomes |
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151 | (12) |
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163 | (9) |
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13 Intracytoplasmic sperm injection: Technical aspects |
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172 | (14) |
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186 | (11) |
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15 Human embryo biopsy procedures |
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197 | (15) |
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16 Analysis of fertilization |
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212 | (6) |
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17 Culture systems for the human embryo |
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218 | (22) |
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18 Evaluation of embryo quality: Analysis of morphology and quantification of nutrient utilization and the metabolome |
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240 | (14) |
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19 Evaluation of embryo quality: Time-lapse imaging to assess embryo morphokinesis |
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254 | (12) |
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20 Evaluation of embryo quality: Proteomic strategies |
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266 | (9) |
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21 The human oocyte: Controlled rate cooling |
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275 | (10) |
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22 The human oocyte: Vitrification |
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285 | (8) |
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23 The human embryo: Slow freezing |
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293 | (14) |
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24 The human embryo: Vitrification |
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307 | (17) |
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VI Diagnosis of Genetic Disease in Preimplantation Embryos |
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25 Severe male factor: Genetic consequences and recommendations for genetic testing |
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324 | (12) |
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26 Polar body biopsy and its clinical application |
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336 | (10) |
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27 Preimplantation genetic diagnosis for infertility |
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346 | (8) |
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28 Genetic analysis of the embryo |
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354 | (12) |
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29 The analysis of endometrial receptivity |
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366 | (14) |
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VIII Future Directions and Clinical Applications |
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30 Human embryonic stem cells |
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380 | (16) |
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31 Microfluidics in ART: Current progress and future directions |
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396 | (19) |
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| Index |
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David K Gardner DPhil is Chair of Zoology at the University of Melbourne, Australia Ariel Weissman MD is a senior physician at the IVF Unit, Department of Obstetrics and Gynecology. Edith Wolfson Medical Center, Holon, and Sackler Faculty of Medicine,Tel Aviv University, Israel Colin M Howles PhD, FRSM is Vice President, Regional Medical Affairs Fertility, Global Business Unit Fertility and Endocrinology, Merck Serono. SA, Geneva, Switzerland Zeev Shoham MD is Director, Reproductive Medicine and Infertility Unit, Department of Obstetrics and Gynecology, at Kaplan Medical Center, Rehovot, Israel
