Established as a definitive reference for the IVF clinic, the fifth edition has been extensively revised, with the addition of several important new contributions on laboratory topics, including advanced sperm selection techniques for ICSI, human embryo biopsy procedures, oocyte activation, managing an oocyte bank, artificial gametes, and epigenetics. As previously, methods, protocols, and techniques of choice are presented by IVF pioneers and eminent international experts.
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vii | |
| The beginnings of human in vitro fertilization |
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xi | |
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| Robert G. Edwards and the thorny path to the birth of Louise Brown: A history of in vitro fertilization and embryo transfer |
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xxv | |
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1 New guidelines for setting up an assisted reproduction technology laboratory |
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1 | (9) |
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2 Quality control: Maintaining stability in the laboratory |
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10 | (7) |
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3 The assisted reproduction technology laboratory: Current standards |
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17 | (19) |
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36 | (14) |
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5 Sperm preparation techniques |
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50 | (15) |
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6 Sperm chromatin assessment |
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65 | (23) |
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7 Oocyte retrieval and selection |
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88 | (20) |
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8 Preparation and evaluation of oocytes for intracytoplasmic sperm injection |
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108 | (9) |
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9 Advanced sperm selection techniques for intracytoplasmic sperm injection |
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117 | (11) |
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10 Use of in vitro maturation in a clinical setting: Patient populations and outcomes |
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128 | (13) |
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11 Intracytoplasmic sperm injection: Technical aspects |
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141 | (15) |
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156 | (12) |
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13 Human embryo biopsy procedures |
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168 | (9) |
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14 Assisted oocyte activation: Current understanding, practice, and future perspectives |
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177 | (16) |
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15 Analysis of fertilization |
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193 | (7) |
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16 Culture systems for the human embryo |
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200 | (25) |
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17 Evaluation of embryo quality: Analysis of morphology and physiology |
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225 | (18) |
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18 Evaluation of embryo quality: Time-lapse imaging to assess embryo morphokinesis |
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243 | (14) |
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19 Evaluation of embryo quality: Proteomic strategies |
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257 | (9) |
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20 The human oocyte: Controlled-rate cooling |
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266 | (14) |
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21 The human oocyte: Vitrification |
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280 | (8) |
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22 The human embryo: Slow freezing |
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288 | (8) |
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23 The human embryo: Vitrification |
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296 | (20) |
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24 Managing an oocyte bank |
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316 | (10) |
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25 Severe male factor infertility: Genetic consequences and recommendations for genetic testing |
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326 | (13) |
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26 Polar body biopsy and its clinical application |
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339 | (11) |
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27 Preimplantation genetic diagnosis for infertility |
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350 | (9) |
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28 Genetic analysis of the embryo |
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359 | (14) |
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29 Diagnosis of endometrial receptivity and the embryo-endometrial dialog |
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373 | (8) |
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30 Artificial gametes: The oocyte |
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381 | (9) |
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31 Microfluidics in assisted reproduction technology: Towards automation of the in vitro fertilization laboratory |
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390 | (20) |
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32 Epigenetic considerations in preimplantation mammalian embryos |
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410 | (5) |
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| Index |
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415 | |
David K. Gardner DPhil Chair of Zoology, University of Melbourne, Melbourne, Australia
Ariel Weissman MD Senior Physician, IVF Unit, Department of Obstetrics and Gynecology, Edith Wolfson Medical Center, Holon and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
Colin M. Howles PhD, FRSM Aries Consulting SARL, Geneva, Switzerland Honorary Fellow, University of Edinburgh, UK
Zeev Shoham MD Director, Reproductive Medicine and Infertility Unit, Department of Obstetrics and Gynecology, Kaplan Medical Center, Rehovot, Israel
