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E-grāmata: Thermomorphogenesis: Methods and Protocols

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  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 2795
  • Izdošanas datums: 09-Apr-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071638149
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  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 2795
  • Izdošanas datums: 09-Apr-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071638149
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This detailed collection explores thermomorphogenesis, a discipline that unravels the intricacies of warm temperature responses in plants. How plants perceive, interpret, and respond to elevated temperatures has far-reaching implications for agriculture, ecology, and our fundamental understanding of plant development, and this book presents techniques to help researchers delve into this area of study. Beginning with a section on thermomorphogenesis responses, the volume continues with numerous chapters on temperature sensing and temperature signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Thermomorphogenesis: Methods and Protocols serves as an ideal guide to this vital subject for research laboratories, as well as for graduate and undergraduate laboratory courses in plant molecular biology.
A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single
Timepoints and by Interval Monitoring.- Dissection of Daytime and Nighttime
Thermoresponsive Hypocotyl Elongation in Arabidopsis.- Light-Dependent High
Ambient Temperature-Induced Senescence Assay Using Whole Seedlings.-
Assessing Temperature Responses in Roots.- A Luciferase Imaging-Based Assay
for Studying Temperature Compensation of the Circadian Clock.- Investigating
the Effects of Temperature on Pathogen Propagation in Arabidopsis.- Assessing
the High Temperature Effects on Stomatal Production.- Characterizing the
Synergistic Response Induced by Warm Temperatures and Shade.- Measurement of
Phytochrome B Thermal Reversion Rates In Vivo.- Characterization of
Thermoresponsive Photobody Dynamics.- Phase Separation of Phytochrome B in
HEK293T Cells.- Isolation of Phytochrome B Photobodies.- Analysis of Phase
Separation of EARLY FLOWERING 3.- In Vitro Determination of
Temperature-Dependent DNA-Binding of the Evening Complex Using
Electrophoretic Mobility Shift Assays.- Monitoring Real-Time Temperature
Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and
Circular Dichroism.- Characterization of PIF4 Phosphorylation by SPA1.-
Chromatin Immunoprecipitation to Investigate H2A.Z Dynamics in Response to
Environmental Changes.- Assessing the Function of CBF1 in Modulating the
Interaction between Phytochrome B and PIF4.- GST Pull-Down Assay to Study
PIF4 Binding In Vitro.- Investigating Circadian Gating of Temperature
Responsive Genes.- RuBisCO Depletion and Subcellular Fractionation for
Enhanced Florigen Detection in Arabidopsis.- Identifying Causal Genes for
Thermo-Responsive Rosette Growth in Arabidopsis by Genome-Wide Association
Study.- Mathematical Modeling of Photo- and Thermomorphogenesis in Plants.