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Tumor Immunology and Immunotherapy Cellular Methods Part A, Volume 631 [Hardback]

Volume editor Lorenzo Galluzzi (Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA), Volume editor Nils-Petter Rudqvist (Postdoctoral Associate in Radiation Oncology, Weill Cornell Medicine, USA)

Formāts: Hardback, 554 pages, height x width: 229x152 mm, weight: 1000 g
Sērija : Methods in Enzymology
Izdošanas datums: 20-Jan-2020
Izdevniecība: Academic Press Inc
ISBN-10: 0128186739
ISBN-13: 9780128186732

Citas grāmatas par šo tēmu:

Molecular biology
Biochemistry - (Noliktavā: 2 punkts)

Hardback
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Formāts: Hardback, 554 pages, height x width: 229x152 mm, weight: 1000 g
Sērija : Methods in Enzymology
Izdošanas datums: 20-Jan-2020
Izdevniecība: Academic Press Inc
ISBN-10: 0128186739
ISBN-13: 9780128186732

Citas grāmatas par šo tēmu:

Molecular biology
Biochemistry - (Noliktavā: 2 punkts)

Permanent link: https://www.kriso.lv/db/9780128186732.html

Keywords:

Tumor Immunology and Immunotherapy - Cellular Methods Part A, Volume 631, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters include Detection of intracellular cytokine production by T cells with flow cytometry, High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers, In vitro assays for effector T cell functions and activity of immunostimulatory antibodies, Ex vivo energetic profiling of tumor cells and T cells from mouse models and human samples, A cytofluorimetric assay to evaluate T cell polyfunctionality, and much more.

Contains the authority of authors who are leaders in their field
Provides a comprehensive source on new methods and research in enzymology

Contributors

Preface: More than two decades of modern tumor immunology

xxiii

1 Cytokine profiling of tumor-infiltrating T lymphocytes by flow cytometry

(20)

Jeremy B. Foote

Sujith Sarvesh

Leisha A. Emens

1 Introduction

(1)

2 Tumor dissociation and T cell isolation

(4)

3 In vitro T cell stimulation

(4)

4 Combining cell surface and intracellular cytokine staining

(4)

5 Data acquisition and analysis

(1)

6 Concluding remarks

(5)

References

(3)

2 High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers

(22)

Morgane Magnin

Philippe Guillaume

George Coukos

Alexandre Harari

Julien Schmidt

1 Introduction

(1)

2 Monitoring of antigen-specific CD8+ and CD4+ T cells with soluble pMHC I & II

(5)

3 Staining of antigen-specific CD8+ and CD4+ T cells: Materials and methods

(3)

4 High dimensional T-cell staining

(4)

5 Reversible pMHC multimer for comprehensive analysis of antigen-specific T cells

(2)

6 Conclusion

(6)

References

(5)

3 In vitro assays for effector T cell functions and activity of immunomodulatory antibodies

(18)

Roberta Zappasodi

Sadna Budhu

Mohsen Abu-Akeel

Taha Merghoub

1 Introduction

(2)

2 Functional assays for T cell co-stimulatory molecules: Principles

(3)

3 Functional assays for T-cell co-stimulatory molecules: Protocol

(4)

4 FACS compensation, set up and data analysis

(4)

5 Concluding remarks

(4)

Acknowledgments

(1)

Competing interests

(1)

References

(3)

4 A cytofluorimetric assay to evaluate T cell polyfunctionality

(16)

Belinda Palermo

Mariangela Panetta

Giulia Campo

Paola Nistico

1 Introduction

(2)

2 Basic principles of intracellular staining

(2)

3 T cells and activation protocols

(3)

4 ICS protocols

(2)

5 Flow cytometric data acquisition and analysis

(2)

6 Concluding remarks

(1)

7 Notes

(4)

Acknowledgments

(1)

References

(2)

5 Assessment of memory formation by metabolically engineered antigen-specific CD8 T cells

(14)

Mathias Wenes

Pedro Romero

Lianjun Zhang

1 Introduction

(2)

2 In vitro assays

(5)

3 In vivo assays

(3)

4 Concluding remarks

(3)

References

(3)

6 Functional characterization of tumor antigen-specific T-cells isolated from the tumor microenvironment of sleeping beauty induced murine glioma models

(16)

Mahmoud S. Alghamri

Felipe J. Nunez

Neha Kamran

Stephen Carney

David Altshuler

Pedro R. Lowenstein

Maria G. Castro

1 Introduction

(2)

2 Generation of glioma neurospheres derived from sleeping beauty transposon mouse glioma model

(2)

3 Stable transfection of neurospheres with a surrogate tumor antigen

(1)

4 Assessment of T-cell function

(6)

5 Summary

103

(4)

Acknowledgments

103

(1)

References

103

(4)

7 Methods to edit T cells for cancer immunotherapy

107

(30)

Francesca Lucibello

Silvia Menegatti

Laurie Menger

1 Introduction

108

(1)

2 TCR-engineered T cells, MHC dependency

109

(5)

3 CAR T-cell, MHC independency

114

(4)

4 Suicide gene strategies

118

(1)

5 Genome-wide CRISPR-Cas9 engineering

119

(1)

6 Generation of CAR T cells using lentiviral strategy

120

(4)

7 Summary

124

(13)

References

124

(13)

8 Generating stem-like memory T cells with antioxidants for adoptive cell transfer immunotherapy of cancer

137

(22)

Karolina Pilipow

Eloise Scamardella

Enrico Lugli

1 Introduction

138

(7)

2 Protocol

145

(6)

3 Analysis

151

(1)

4 Summary

152

(7)

Acknowledgments

154

(1)

References

154

(5)

9 Reverse immunology: From peptide sequence to tumor-killing human T-cell clones

159

(36)

Christophe Vanhaver

Monica Gordon-Alonso

Alexandre Bayard

Maria Teresa Catanese

Didier Colau

Pierre van der Bruggen

Annika M. Bruger

1 Introduction

160

(2)

2 Identification of peptides binding to HLA molecules

162

(4)

3 Isolation of peptide-specific T-cell clones

166

(16)

4 Validation steps to identify tumor-killing T-cell clones

182

(5)

5 T-cell functional assays

187

(4)

6 Concluding remarks

191

(4)

Acknowledgments

191

(1)

References

191

(3)

Further reading

221

(2)

11 In vitro expansion of Vγ9Vδ2 T cells for immunotherapy

223

(16)

Christian Peters

Leonce Kouakanou

Hans-Heinrich Oberg

Daniela Wesch

Dieter Kabelitz

1 Introduction

224

(5)

2 Protocol for the Vγ9Vδ2 T-cell expansion at laboratory scale

229

(4)

3 Adaptations for the clinical application

233

(6)

References

235

(4)

12 CFSE dilution to study human T and NK cell proliferation in vitro

239

(18)

Inigo Terren

Ane Orrantia

Joana Vitalle

Olatz Zenarruzabeitia

Francisco Borrego

1 Introduction

240

(3)

2 CFSE staining and cell culture

243

(1)

3 Flow cytometry staining

244

(1)

4 Proliferation analysis

245

(4)

5 Concluding remarks

249

(2)

6 Notes

251

(6)

Acknowledgments

253

(1)

Conflict of interests

253

(1)

References

253

(4)

13 Rapid isolation and enrichment of mouse NK cells for experimental purposes

257

(20)

Maite Alvarez

Maria C. Ochoa

Luna Minute

Ignacio Melero

Pedro Berraondo

1 Introduction

258

(2)

2 IL-15

260

(1)

3 Sushi-IL 15-Apo construction

261

(2)

4 Hydrodynamic delivery

263

(1)

5 NK cell expansion and isolation protocol

264

(2)

6 NK cell characterization

266

(5)

7 Conclusions

271

(6)

Acknowledgments

272

(1)

Conflict of interest disclosure

272

(1)

References

272

(5)

14 Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood

277

(12)

Peter Holicek

Iva Truxova

Lenka Kasikova

Sarka Vosahlikova

Cyril Salek

Jana Rakova

Monika Holubova

Daniel Lysak

Isabelle Cremer

Radek Spisek

Jitka Fucikova

1 Introduction

278

(1)

2 NK cell isolation: Principle

278

(1)

3 NK cell isolation: Protocol

279

(1)

4 NK cell cytotoxicity: Principles

280

(1)

5 NK Cell cytotoxicity: Protocols

281

(3)

6 Concluding remarks

284

(2)

7 Notes

286

(3)

Acknowledgments

286

(1)

References

287

(2)

15 Two-dimensional dynamic evaluation of natural killer cell-mediated lysis of adherent target cells

289

(16)

Alexandra Frazao

Louise Rethacker

Anne Caignard

1 Introduction

290

(4)

2 Assessment of NK cell-mediated lysis using cell impedance dynamic measure

294

(6)

3 Concluding remarks

300

(1)

4 Notes

301

(4)

Acknowledgments

301

(1)

References

301

(4)

16 Rapid isolation of mouse ILCs from murine intestinal tissues

305

(24)

Kyle Burrows

Pailin Chiaranunt

Louis Ngai

Arthur Mortha

1 Introduction

306

(6)

2 Identification of gut innate lymphoid cell populations by flow cytometry

312

(2)

3 Protocol

314

(15)

Acknowledgments

323

(1)

References

323

(6)

17 Detecting and analyzing murine innate lymphoid cells

329

(14)

Alejandra Gomez-Cadena

Pedro Romero

Sara Trabanelli

Camilla Jandus

1 Introduction

330

(1)

2 Identification, isolation and stimulation of murine ILCs

331

(12)

References

342

(1)

18 A cytofluorimetric assay to evaluate intracellular cytokine production by NK cells

343

(14)

Christian Sordo-Bahamonde

Seila Lorenzo-Herrero

Segundo Gonzalez

Alejandro Lopez-Soto

1 Introduction

344

(1)

2 Materials and methods

345

(2)

3 Assessment of cytokine production in NK cells by intracellular staining

347

(3)

4 Notes

350

(7)

References

354

(3)

19 Applications of microfluidic devices in advancing NK-cell migration studies

357

(14)

Xiaoou Ren

Abdulaziz Alamri

Jolly Hipolito

Francis Lin

Sam K.P. Kung

1 Introduction

358

(2)

2 Preparation of microfluidic devices

360

(3)

3 Generation of chemoattractant gradients

363

(1)

4 Preparations of cells

363

(1)

5 Experimental setup

364

(2)

6 Data analysis

366

(2)

7 Conclusion

368

(3)

Acknowledgments

369

(1)

References

369

(2)

20 Complementary approaches to study NKT cells in cancer

371

(20)

Jay A. Berzofsky

Purevdorj B. Olkhanud

Masaki Terabe

1 Introduction

372

(2)

2 Type I NKT cells

374

(4)

3 Type II NKT cells

378

(2)

4 NKT cells in cancer

380

(1)

5 Type II NKT cells

381

(3)

6 Conclusion

384

(7)

References

384

(7)

21 Assessment of IFN-y and granzyme-B production by in "sitro" technology

391

(24)

Claudia Galassi

Gwenola Manic

Martina Musella

Antonella Sistigu

Ilio Vitale

1 Introduction

393

(3)

2 Materials

396

(3)

3 Methods

399

(7)

4 Discussion and concluding remarks

406

(9)

Notes

407

(4)

Acknowledgments

411

(1)

References

411

(4)

22 Assessment of IFNy responsiveness in patient-derived xenografts

415

(14)

Jordan J. Cardenas

Camila Robles-Oteiza

Katerina Politi

1 Introduction

416

(1)

2 Establishment and maintenance of PDX lines

417

(2)

3 In vivo stimulation of PDX lines with interferon gamma

419

(1)

4 Protocol

420

(3)

5 Analysis

423

(3)

6 Summary

426

(3)

Acknowledgments

426

(1)

References

426

(3)

23 Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitro

429

(14)

Hans-Heinrich Oberg

Christian Peters

Dieter Kabelitz

Daniela Wesch

1 Introduction

430

(1)

2 Tumor cell lines

431

(1)

3 Effector cells

432

(2)

4 Protocol

434

(1)

5 RTCA-system

435

(5)

6 Summary

440

(3)

Acknowledgments

440

(1)

References

440

(3)

24 Analysis of cancer cell-intrinsic immune regulation in response to CD8+ T cell attack

443

(24)

Natalie J. Neubert

Laure Tille

Christophe Martignier

Silvia A. Fuertes

Marraco

Daniel E. Speiser

1 Introduction

444

(1)

2 Methods

445

(3)

3 Selection of melanoma cell lines and CD8+ cytotoxic T lymphocyte (CTL) clones

448

(2)

4 Establishment of the melanoma cell/T cell co-culture

450

(2)

5 CTL and melanoma cell viability and ratio

452

(2)

6 CTL function

454

(1)

7 Kinetic of protein expression

454

(1)

8 mRNA screen of co-cultured melanoma cells

454

(3)

9 Assessment of HLA upregulation in a larger melanoma cell panel

457

(3)

10 Discussion and conclusion

460

(7)

Acknowledgments

464

(1)

Conflict of interest

464

(1)

References

464

(3)

25 A flow cytometry-based method to screen for modulators of tumor-specific T cell cytotoxicity

467

(16)

Javier Santos

Jesus Ogando

Rosa Ana Lacalle

Santos Manes

1 Introduction

468

(1)

2 From CD8+ T lymphocytes to armed CTL

469

(1)

3 Suppression of the CTL response by the PD-1/PD-L1 axis

470

(1)

4 Overview of the CTL cytotoxicity assay

471

(1)

5 Generation of stable E.G7-OVA-PD-L1-expressing cells

472

(2)

6 Generation of ex vivo-activated OT-I cells

474

(2)

7 Cytotoxicity assay

476

(1)

8 Concluding remarks

477

(6)

Acknowledgments

480

(1)

References

480

(3)

26 Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay

483

(14)

Seila Lorenzo-Herrero

Christian Sordo-Bahamonde

Segundo Gonzalez

Alejandro Lopez-Soto

1 Introduction

484

(1)

2 Cell culture

485

(1)

3 Isolation of peripheral blood mononuclear cells (PBMCs)

486

(1)

4 Target cell labeling

487

(1)

5 Effector/target cell co-culture

487

(1)

6 Fluorometric analysis

488

(2)

7 Concluding remarks

490

(1)

8 Notes

490

(7)

Acknowledgments

494

(1)

References

494

(3)

27 51Cr-release to monitor NK cell cytotoxicity

497

Leslie Eisner

Ralf Dressel

1 Introduction

498

(2)

2 Cell culture of target cells

500

(1)

3 Isolation of human NK cells

501

(1)

4 Isolation of murine NK cells

501

(1)

5 Activation of NK cells

502

(1)

6 51Cr-release assay

503

(3)

7 Data analysis

506

(1)

8 Concluding remarks

507

(1)

9 Notes

507

Acknowledgments

511

(1)

References

511

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.

Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.

Tumor Immunology and Immunotherapy Cellular Methods Part A, Volume 631 [Hardback]

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