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Zero to Genetic Engineering Hero 2e [Mīkstie vāki]

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  • Formāts: Paperback / softback, 200 pages, height x width x depth: 274x216x11 mm, weight: 514 g
  • Izdošanas datums: 27-Aug-2021
  • Izdevniecība: Make Community, LLC
  • ISBN-10: 1680457160
  • ISBN-13: 9781680457162
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Zero to Genetic Engineering Hero 2ePalielināt
  • Formāts: Paperback / softback, 200 pages, height x width x depth: 274x216x11 mm, weight: 514 g
  • Izdošanas datums: 27-Aug-2021
  • Izdevniecība: Make Community, LLC
  • ISBN-10: 1680457160
  • ISBN-13: 9781680457162
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781680457162.html
Keywords:

Zero to Genetic Engineering Hero is made to provide you with a first glimpse of the inner-workings of a cell. It further focuses on skill-building for genetic engineering and the Biology-as-a-Technology mindset (BAAT). This book is designed and written for hands-on learners who have little knowledge of biology or genetic engineering. This book focuses on the reader mastering the necessary skills of genetic engineering while learning about cells and how they function. The goal of this book is to take you from no prior biology and genetic engineering knowledge toward a basic understanding of how a cell functions, and how they are engineered, all while building the skills needed to do so.

Authors 6(1)
Junior Editors 7(1)
Preface 8(3)
Who this book is for
9(1)
This book is not a typical textbook!
9(1)
Acknowledgements
9(2)
The Genetic Engineers' Pledge
11(1)
Isolating DNA, the Blueprints of Life
12(27)
Equipment and Materials
13(2)
Learning Hands-On: Breaking Cells Open & Extracting DNA
15(1)
Step 1 Create salt water inside the resealable bag
15(1)
Step 2 Mashing the strawberry into individual cells
16(1)
Step 3 Breaking open the cells with soap
17(2)
Step 4 Filtering the cell debris
19(1)
Step 5 Precipitating the DNA
19(4)
Fundamentals: DNA
23(1)
Evolution: It's natural for DNA to change
23(1)
Modern Synthesis
24(2)
Genetic engineering: The road to precise editing of DNA
26(1)
Atoms, molecules, and macromolecules of the cell
27(5)
Understanding the nomenclature of DNA
32(2)
DNA extractions in the real-world
34(2)
Summary and What's Next?
36(3)
Setting Up Your Genetic Engineering Hero Space
39(12)
Getting Started
10(30)
Do I need government approval?
40(1)
What type of room should I set up my genetic engineering space in?
41(1)
Equipment and materials for your Genetic Engineering Hero space
42(2)
Materials and Supplies
44(1)
Cleaning and Other Supplies
45(1)
Experiment kits/wetware
46(1)
Safety and Best Practices
46(1)
Just because I Can, does it mean I should?
47(4)
Growing E. coli Cells
51(35)
Getting Started
53(1)
Equipment and Materials
53(1)
Virtual Bioengineer™ simulation Breakout Session 2
54(1)
Learning Hands-On: Growing K12 E. coli cells
55(1)
Step 1 Download the instruction manual for the Canvas Kit
55(1)
Step 2 Put on your gloves and lab coat
55(1)
Step 3 Create molten LB agar powder
56(1)
Step 4 Adding antibiotic
57(1)
Step 5 Pour LB agar plates
58(2)
Step 6 Use & storage of LB agar plates
60(1)
Step 7 Streaking E. coli bacteria
61(1)
Step 8 Incubating E. coli cells
61(1)
Step 9 Viewing plates of grown bacteria
62(1)
Step 10 Painting living art (bioart) with R coli bacteria
63(1)
Step 11 Incubating E. coli cells
64(1)
Step 12 Viewing & Preserving your bioart with a Keep-it Kit
64(1)
Step 13 Clean-up and inactivation
65(2)
Fundamentals: E. coli Cells
67(1)
Introduction to "Lab" E. coli
67(3)
A Tour of the E. coli Microfactory
70(1)
The Fence (A)
70(2)
The Outer Wall (B)
72(2)
The Lobby (C)
74(1)
The Inner Walls (D)
75(1)
The Factory Floor (E)
75(8)
Summary and What's Next?
83(3)
Genetic Engineering Your E. coli Cells
86(33)
Getting Started
87(1)
Equipment and Materials
87(2)
Learning Hands-On: Transforming K12 E. coli cells with a DNA Plasmid
89(1)
Step 1 Download the instruction manual for the Engineer-it Kit
89(1)
Step 2 Put on your gloves and lab coat
89(1)
Step 3 Label your plates
90(1)
Step 4 Make non-selective and selective LB agar plates
90(2)
Step 5 Streaking E. coli and the negative control plates
92(2)
Checkpoint!
94(1)
Step 6 Making chemically competent cells
95(1)
Step 7 Add DNA plasmids and Heat Shock
96(2)
Step 8 Recovery step
98(1)
Step 9 Plating and incubating your cells
99(1)
Step 10 What to expect & inactivation
100(1)
Fundamentals: How a cell reads a DNA plasmid
101(1)
The basic operating environment of a cell: The Four B's
101(1)
(Bump, Bind, Burst, Bump)
101(1)
The Three Steps to Microfacturing
102(1)
Deoxyribonucleic acid (DNA) vs. Ribonucleic acid (RNA)
103(2)
RNA polymerase: The cell machine that transcribes
105(1)
What is a gene?
106(1)
Starting Transcription
106(3)
During transcription: Direction
109(1)
During transcription: Which DNA strand does RNA polymerase read?
110(2)
During transcription: A secret cipher for transcribing DNA to RNA
112(1)
Stopping transcription
113(1)
What can you do with RNA?
114(2)
Summary and What's Next?
116(3)
Extracting your engineered proteins
119(28)
Getting Started
120(1)
Equipment and Materials
120(1)
Learning Hands-On: Culture and lyse engineered E. coli to obtain a protein product extract
121(1)
Step 1 Download the instruction manual for the Plate Extract-it Kit
121(1)
Step 2 Put on your gloves and lab coat
121(1)
Step 3 Transform cells to get fresh colonies (Optional)
121(1)
Step 4 Make selective LB agar plates for amplification
122(1)
Step 5 Culturing: Spread out your freshly engineered cells
122(1)
Step 6 Culturing: Incubate at 37 °C for 24-48 hours
123(2)
Step 7 Extraction: Collect cells and start the lysis
125(1)
Step 8 Extraction: Lyse the cells
126(1)
Step 9 Extraction: Pellet the cell debris
127(1)
Step 10 Extraction: Filter sterilize your proteins
128(1)
Step 11 Using your proteins
129(2)
Fundamentals: How cells translate proteins from RNA
131(1)
Step two of the Three Steps to Microfacturing: Translating proteins from RNA
131(1)
Starting Translation
132(1)
During Translation: The RNA to protein cipher
133(4)
During Translation: Locating the starting point for translation
137(2)
Stopping Translation
139(3)
Summary and What's Next?
142(3)
Check Point!
145(2)
Processing Enzymes
147(30)
Getting Started
149(1)
Equipment and Materials
149(1)
Learning Hands-On: Process one molecule into another using enzymes
150(1)
Exercise 1 Enzymatic processing to generate smells
150(1)
Step 1 Download the instruction manual for the Smell-it Kit
150(1)
Step 2 Complete the engineering part of the Smell-it Kit
150(1)
Step 3 Culture cells with the substrate
150(1)
Step 4 Labeling and creating your LB agar plates for culturing
151(1)
Step 5 Culture engineered cells on your selective LB agar plate
152(1)
Exercise 2 Enzymatic processing to generate color
153(1)
Step 1 Download the instruction manual for the Blue-it Kit
154(1)
Step 2 Complete genetic engineering and extraction procedures
154(1)
Step 3 Dissolve your substrates
154(1)
Step 4 Add cell extract beta-galactosidase to the substrate
155(4)
Fundamentals: Diving into enzymatic processing
159(1)
The basics of enzymatic chemical reactions
159(1)
The Four B's and enzyme function
159(2)
Atoms
161(2)
Bonds
163(5)
Protein enzyme catalysis in cells
168(6)
Summary and What's Next?
174(3)
Manually turning on genes in situ
177(15)
Getting Started
178(1)
Equipment and Materials
178(1)
Learning Hands-On: Manually turning on genes in situ
179(1)
Exercise 1 Inducing a gene using a chemical
179(1)
Step 1 Complete the Induce-it Kit engineering exercise
179(1)
Step 2 Culture your cells
179(1)
Step 3 Add your inducer
179(1)
Exercise 2 Inducing a gene using temperature
180(1)
Step 1 Complete the Heat-it Kit engineering
180(1)
Step 2 Increase the temperature
181(1)
Exercise 3 Inducing a gene using light
181(1)
Step 1 Streak cells
181(1)
Step 2 Turn on the light!
181(2)
Fundamentals: Diving deeper into genetic `switches'
183(1)
Turn on genes with chemicals
183(2)
Turn on genes with temperature
185(1)
Turn on genes with light
186(3)
Summary
189(2)
Periodic table 192
Dr. Justin Pahara has more than a decade of bioengineering experience as well as extensive knowledge of synthetic biology tech, markets, and work-flows. Justin studied Synthetic Biology at the University of Cambridge (PhD, MoTI in JBS), Singularity University (GSP-10; Google Fellow), iGEM (2007, 2008), the University of Alberta (B.Sc., M.Sc.).

Julie Legault is a designer-entrepreneur from the city of Montreal. Over the last decade, Julie has worked in design research, innovation and the maker movement. Julie began her career earning a B.F.A. (Design & Computation Art) and a Graduate Certificate (Digital Technologies in Design Art) from Concordia University in Montreal. Julie furthered her skills and knowledge by completing a Master of Art in the School of Materials at the Royal College of Art in London, United Kingdom and a Master of Science at the Massachusetts Institute of Technology (MIT) Media Lab. Grateful for her early access to computing in her childhood thanks to a pioneering mother, Julie has since dedicated herself to translating complex technologies for beginners through teaching and applied design.