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Adult Stem Cells: Methods and Protocols 1st ed. 2017 [Hardback]

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  • Formāts: Hardback, 324 pages, height x width: 254x178 mm, weight: 1106 g, 54 Illustrations, color; 9 Illustrations, black and white; XII, 324 p. 63 illus., 54 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 1553
  • Izdošanas datums: 25-Feb-2017
  • Izdevniecība: Humana Press Inc.
  • ISBN-10: 1493967541
  • ISBN-13: 9781493967544
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  • Formāts: Hardback, 324 pages, height x width: 254x178 mm, weight: 1106 g, 54 Illustrations, color; 9 Illustrations, black and white; XII, 324 p. 63 illus., 54 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 1553
  • Izdošanas datums: 25-Feb-2017
  • Izdevniecība: Humana Press Inc.
  • ISBN-10: 1493967541
  • ISBN-13: 9781493967544
Citas grāmatas par šo tēmu:

This volume contains a collection of protocols from some of the major laboratories involved in stem cell research across the world. The research discussed in this book covers topics such as: isolating, characterizing and expanding dental pulp stem cells; manipulating the proliferative potential of cardiomyocytes by gene transfer; isolation of stromal stem cells from adipose tissue; noninvasive assessment of cell fat and biology in transplanted mesenchymal stem cells; and cell-free therapy for organ repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Adult Stem Cells: Methods and Protocols is a valuable resource for helping researchers transform the study of stem cells into an industrialized process that will supply patients with efficient, safe, and cost-effective cell treatments.

Preface v
Contributors ix
1 A Simple Protocol to Isolate, Characterize, and Expand Dental Pulp Stem Cells
1(14)
Federica Di Scipio
Andrea Elio Sprio
Maria Elisabetta Carere
Zhiqian Tang
Giovanni Nicolao Berta
2 Derivation of Mesenchymal Stem Cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells
15(10)
Glen Lester Sequiera
Niketa Sareen
Ejlal Abu El-Rub
Sanjiv Dhingra
3 Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren's-Like Disease in NOD Mice
25(16)
Ghada Abu Elghanam
Younan Liu
Saeed Khalili
Dongdong Fang
Simon D. Tran
4 Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer
41(14)
Giulia Prosdocimo
Mauro Giacca
5 Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells
55(12)
Ghazaleh Hajmousa
Martin C. Harmsen
6 Isolation and In Vitro Characterization of Epidermal Stem Cells
67(18)
Kasper S. Moestrup
Marianne S. Andersen
Kim B. Jensen
7 Endothelial Progenitor Cells: Procedure for Cell Isolation and Applications
85(6)
Garikipati V.N. Srikanth
Raj Kishore
8 Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID
91(24)
Vikram Sabapathy
Franklin Jebaraj Herbert
Sanjay Kumar
9 Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID
115(18)
Vikram Sabapathy
Balasubramanian Sundaram
Sanjay Kumar
10 Isolation, Characterization, and Expansion of Cancer Stem Cells
133(12)
Luke A. Torre-Healy
Artem Berezovsky
Justin D. Lathia
11 Cardiac Progenitor Cell Extraction from Human Auricles
145(10)
Paolo Di Nardo
Francesca Pagliari
12 Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle
155(14)
Antonio Musaro
Silvia Carosio
13 Isolation of Stromal Stem Cells from Adipose Tissue
169(14)
Maria Prat
Francesca Oltolina
Silvia Antonini
Andrea Zamperone
14 Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells as an Individual-Specific and Renewable Source of Adult Stem Cells
183(8)
Glen Lester Sequiera
Sekaran Saravanan
Sanjiv Dhingra
15 A Simplified and Systematic Method to Isolate, Culture, and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth
191(18)
Mohammed Bakkar
Younan Liu
Dongdong Vang
Camille Stegen
Xinyun Su
Murali Ramamoorthi
Li-Chieh Lin
Takako Kawasaki
Nicholas Makhoul
Huan Pham
Yoshinori Sumita
Simon D. Tran
16 Measurement of Autophagy by Flow Cytometry
209(8)
Silvia Zappavigna
Angela Lombardi
Gabriella Misso
Anna Grimaldi
Michele Caraglia
17 Echocardiography-Guided Intramyocardial Injection Method in a Murine Model
217(10)
Kay Maeda
Rick Seymour
Marc Ruel
Erik J. Suuronen
18 Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells
227(14)
Federico Franchi
Martin Rodriguez-Porcel
19 Methods for Long-Term Storage of Murine Bone Marrow-Derived Mesenchymal Stem Cells
241(8)
Niketa Sareen
Ejlal Abu-El-Rub
Glen Lester Sequiera
Meenal Moudgil
Sanjiv Dhingra
20 Therapeutic Application of Adult Stem Cells in the Heart
249(16)
Taylor A. Johnson
Dinender K. Singla
21 Patient-Derived and Intraoperatively Formed Biomaterial for Tissue Engineering
265(8)
Shalmli U. Joshi
Rares O. Barbu
Melissa Carr-Reynolds
Brian Barnes
Syam P. Nukavarapu
22 Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering
273(20)
Dongdong Fang
Michael Roskies
Mohamed-Nur Abdallah
Mohammed Bakkar
Jack Jordan
Li-Chieh Lin
Faleh Tamimi
Simon D. Tran
23 Optimal Environmental Stiffness for Stem Cell Mediated Ischemic Myocardium Repair
293(12)
Honghai Liu
Christian Paul
Meifeng Xu
24 Assessment of MiRNA Regulation of Endothelial Progenitor Cell Mediated Angiogenesis
305(10)
Darukeshwara Joladarashi
Prasanna Krishnamurthy
25 Stem Cell Exosomes: Cell-FreeTherapy for Organ Repair
315
Mohsin Khan
Raj Kishore