This volume contains a collection of protocols from some of the major laboratories involved in stem cell research across the world. The research discussed in this book covers topics such as: isolating, characterizing and expanding dental pulp stem cells; manipulating the proliferative potential of cardiomyocytes by gene transfer; isolation of stromal stem cells from adipose tissue; noninvasive assessment of cell fat and biology in transplanted mesenchymal stem cells; and cell-free therapy for organ repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Adult Stem Cells: Methods and Protocols is a valuable resource for helping researchers transform the study of stem cells into an industrialized process that will supply patients with efficient, safe, and cost-effective cell treatments.
A Simple Protocol to Isolate, Characterize and Expand Dental Pulp Stem
Cells.- Derivation of Mesenchymal Stem cells from Embryonic Stem Cells: A
Non-Variable and Inexhaustive Source of Adult Stem Cells.- Compact
Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment
of Sjogrens-Like Disease in NOD Mice.- Manipulating the Proliferative
Potential of Cardiomyocytes by Gene Transfer.- Assessment of Energy Metabolic
Changes in Adipose Tissue-Derived Stem Cells.- Isolation and In Vitro
Characterization of Epidermal Stem Cells.- Endothelial Progenitor Cells:
Procedure for Cell Isolation and Characterization.- Therapeutic Application
of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord
Injury of SCID.- Therapeutic Application of Human Wharton Jelly Mesenchymal
Stem Cells in Skin Injury of SCID.- Isolation, Characterization, and
Expansion of Cancer Stem Cells.- Cardiac Progenitor Cell Isolation from Human
Auricles.- Isolation and Culture of Satellite Cells from Mouse Skeletal
Muscle.- Isolation of Stromal Stem Cells from Adipose Tissue.- Human Induced
Pluripotent Stem Cell-Derived Mesencymal Stem Cells as an Individual-Specific
and Renewable Source of Adult Stem Cells.- A Simplified and Systematic Method
to Isolate, Culture and Characterize Multiple Types of Human Dental Stem
Cells from a Single Tooth.- Measurement of Autophagy by Flow
Cytometry.- Echocardiography-Guided Intramyocardial Injection Method in a
Murine Model.- Noninvasive Assessment of Cell Fate and Biology
in Transplanted Mesenchymal Stem Cells.- Methods for Long Term Storage of
Murine Bone Marrow Derived Mesenchymal Stem Cells.- Therapeutic Application
of Adult Stem Cells in the Heart.- Patient-Derived and Intra-Operatively
Formed Biomaterial for Tissue Engineering.- Three-Dimensional Printed
Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular
Reconstruction and Engineering.- Optimal Environmental Stiffness for Stem
Cell Mediated Ischemic Myocardium Repair.- Assessment of MiRNA Regulation of
Endothelial Progenitor Cell Mediated Angiogenesis.- Stem Cell Exosomes: Cell
Free-Therapy for Organ Repair.
