E-grāmata: Antibiotics: Methods and Protocols

Antibiotics - precious goods in changing times.- A Whole-Cell Assay for
Detection of Antibacterial Activity in Actinomycetes Culture
Supernatants.- Sampling of Human Microbiomes to Screen for
Antibiotic-Producing Commensals.- Production of Antimicrobial Compounds by
Homologous and Heterologous Expression.- Isolation and Purification of
Natural Products from Microbial Cultures.- Structure Elucidation of
Antibiotics.- Computer-Aided Drug Design An update.- Cytotoxicity Assays as
Predictors of the Safety and Efficacy of Antimicrobial
Agents.- Microscopy-based Multiwell Assay to Characterize Disturbed Bacterial
Morphogenesis Upon Antibiotic Action.- Expansion Microscopy of Bacillus
subtilis.- Tracking global and local changes in membrane fluidity through
fluorescence spectroscopy and microscopy.- Quantitative analysis of
microscopy data to evaluate bacterial responses to antibiotic
treatment.- Application of a Bacillussubtilis whole-cell biosensor
(PliaI-lux) for the identification of cell wall active antibacterial
compounds.- Determination of Bacterial Membrane Impairment by Antimicrobial
Agents.- A colorimetric assay to identify and characterize bacterial primase
inhibitors.- Cell-based fluorescent screen amenable to HTS to Identify
Inhibitors of Bacterial Translation Initiation.- ­Bacterial two component
systems: Overexpression, purification in vitro and in vivo inhibitor
screens.- Sample preparation for mass-spectrometry based absolute
quantification of bacterial proteins in antibiotic stress
research.- Elemental analysis for the characterization of antimicrobial
effects.- label-free quantitation of ribosomal proteins from bacillus
subtilis for antibiotic research.- Functional Metagenomics to Study
Antibiotic Resistance.