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Antibiotics: Methods and Protocols Second Edition 2023 [Mīkstie vāki]

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  • Formāts: Paperback / softback, 405 pages, height x width: 254x178 mm, weight: 795 g, 62 Illustrations, color; 24 Illustrations, black and white; XII, 405 p. 86 illus., 62 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2601
  • Izdošanas datums: 30-Nov-2023
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071628577
  • ISBN-13: 9781071628577
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  • Mīkstie vāki
  • Cena: 140,62 €*
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  • Formāts: Paperback / softback, 405 pages, height x width: 254x178 mm, weight: 795 g, 62 Illustrations, color; 24 Illustrations, black and white; XII, 405 p. 86 illus., 62 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2601
  • Izdošanas datums: 30-Nov-2023
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071628577
  • ISBN-13: 9781071628577
Citas grāmatas par šo tēmu:

This second edition provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies. Antibiotics: Methods and Protocols,  Second Edition, guides readers through updated and entirely new chapters on production and design, mode of action, and response and resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Antibiotics: Methods and Protocols, Second Edition aims to inspire scientific work in the exciting field of antibiotic research.

Antibiotics - precious goods in changing times.- A Whole-Cell Assay for
Detection of Antibacterial Activity in Actinomycetes Culture
Supernatants.- Sampling of Human Microbiomes to Screen for
Antibiotic-Producing Commensals.- Production of Antimicrobial Compounds by
Homologous and Heterologous Expression.- Isolation and Purification of
Natural Products from Microbial Cultures.- Structure Elucidation of
Antibiotics.- Computer-Aided Drug Design An update.- Cytotoxicity Assays as
Predictors of the Safety and Efficacy of Antimicrobial
Agents.- Microscopy-based Multiwell Assay to Characterize Disturbed Bacterial
Morphogenesis Upon Antibiotic Action.- Expansion Microscopy of Bacillus
subtilis.- Tracking global and local changes in membrane fluidity through
fluorescence spectroscopy and microscopy.- Quantitative analysis of
microscopy data to evaluate bacterial responses to antibiotic
treatment.- Application of a Bacillussubtilis whole-cell biosensor
(PliaI-lux) for the identification of cell wall active antibacterial
compounds.- Determination of Bacterial Membrane Impairment by Antimicrobial
Agents.- A colorimetric assay to identify and characterize bacterial primase
inhibitors.- Cell-based fluorescent screen amenable to HTS to Identify
Inhibitors of Bacterial Translation Initiation.- ­Bacterial two component
systems: Overexpression, purification in vitro and in vivo inhibitor
screens.- Sample preparation for mass-spectrometry based absolute
quantification of bacterial proteins in antibiotic stress
research.- Elemental analysis for the characterization of antimicrobial
effects.- label-free quantitation of ribosomal proteins from bacillus
subtilis for antibiotic research.- Functional Metagenomics to Study
Antibiotic Resistance.