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Biosafety Assessment of Probiotic Potential Second Edition 2025 [Hardback]

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  • Formāts: Hardback, 295 pages, height x width: 254x178 mm, 24 Illustrations, color; 1 Illustrations, black and white; XVI, 295 p. 25 illus., 24 illus. in color., 1 Hardback
  • Sērija : Methods and Protocols in Food Science
  • Izdošanas datums: 20-Sep-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071647571
  • ISBN-13: 9781071647578
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Biosafety Assessment of Probiotic Potential Second Edition 2025Palielināt
  • Formāts: Hardback, 295 pages, height x width: 254x178 mm, 24 Illustrations, color; 1 Illustrations, black and white; XVI, 295 p. 25 illus., 24 illus. in color., 1 Hardback
  • Sērija : Methods and Protocols in Food Science
  • Izdošanas datums: 20-Sep-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071647571
  • ISBN-13: 9781071647578
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781071647578.html

This second edition details the techniques involved in the biosafety assessment of probiotics to be used for human consumption. New and updated chapters guide readers through identification of antibiotic resistance and virulence genes using various databases, PCR techniques, in vitro cytotoxicity assays, cytolysins, and in vivo toxicity assays such as acute and chronic toxicity, virulence, toxigenicity and dermonecrotic effects.  Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols.

Authoritative and cutting-edge, Biosafety Assessment of Probiotic Potential, Second Eidition aims to be comprehensive guide for researchers in the field.

Detection of virulence and antibiotic resistance associated genes by
PCR.- Determination of antibiotic resistance by turbidometric method.-
Determination of Cytotoxicity of Probiotics by Cell Viability Assays.-
Determination of cytotoxicity by lacta dehydrogenase activity.- Determination
of Cytotoxicity by Calcein-Propidium Iodide Staining for live and dead cell
detection.- Determination of Cytotoxicity by Edu Cell Proliferation Assay.-
Detection of Biofilm Formation.- In vitro assessment of cytolysins production
by hemolysis assays.- In vitro assessment of cytolysins genes by PCR.- In
vitro assessment of cytolysin proteins by ELISA.- In vitro assessment of
cytolysin proteins by Western Blotting.- In vitro assessment of cytolysins
production by cytotoxicity assays.- In vitro assessment of cytolysins
production by Mass Spectrometry.- In vivo assessment of cytolysins
production.- Identification of Antibiotic Resistance Genes Using the
Comprehensive Antibiotic Resistance Database (CARD).- Identification of
virulence genes through virulence factor database (VFDB).- Determination of
assessment of genes related to Biogenic Amines through Rapid Annotations
using Subsystems Technology (RAST).- In vivo assessment of microbial
translocation.- In vivo determination of acute oral toxicity.- In vivo
determination of virulence.- In vivo determination of toxigenicity.- In vivo
determination of acute and chronic toxicity.- In vivo determination of
dermonecrotic properties.- In vivo assessment of genotoxicity by Mouse
Spermatogonial Chromosomal Aberration Test.- In vivo assessment of
genotoxicity using COMET assay.- In vitro Cyto-genotoxicity assessment of
probiotics, using cytokinesis-block micronucleus (CBMN) assay in grown
peripheral human lymphocytes.- In vivo assessment of genotoxicity of
probiotics by Mammalian Erythrocyte Micronucleus Test.