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Chromaffin Cells: Methods and Protocols 2023 ed. [Mīkstie vāki]

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  • Formāts: Paperback / softback, 373 pages, height x width: 254x178 mm, weight: 736 g, 68 Illustrations, color; 21 Illustrations, black and white; XIV, 373 p. 89 illus., 68 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2565
  • Izdošanas datums: 09-Oct-2023
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071626736
  • ISBN-13: 9781071626733
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  • Mīkstie vāki
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Chromaffin Cells: Methods and Protocols 2023 ed.Palielināt
  • Formāts: Paperback / softback, 373 pages, height x width: 254x178 mm, weight: 736 g, 68 Illustrations, color; 21 Illustrations, black and white; XIV, 373 p. 89 illus., 68 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2565
  • Izdošanas datums: 09-Oct-2023
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071626736
  • ISBN-13: 9781071626733
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781071626733.html
Keywords:
This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.





 





Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 
Rat pheochromocytoma PC12 cells in culture.- Adrenal grafts in the
central nervous system: Chromaffin and chromaffin progenitor cell
transplantation.- Immunocytochemistry of acutely isolated adrenal medullary
chromaffin cells.- Transmission electron microscopy: a method for studying
the adrenochromaffin cells.- Immunogold for protein location in chromaffin
cells.- Visualization of exo- and endocytosis membrane dynamics with
super-resolution STED microscopy.- Online detection of catecholamine release
from the perfused rat adrenal gland.- On-line recording of perifused
chromaffin cells.- Recording of chromaffin cell electrical activity in situ
in acute adrenal slices.- Calcium imaging and amperometric recording in
cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto
rats, and Spontaneously Hypertensive Rats.- Measurements of calcium in
chromaffin cell organelles using targeted aequorins.- Quantification of
secretory granule exocytosis by TIRF imaging and capacitance
measurements.- Membrane capacitance measurements of stimulus-evoked
exocytosis in adrenal chromaffin cell.- Quantal release analysis of
electrochemically active molecules using single-cell
amperometry.- Methodologies for detecting quantal exocytosis in adrenal
chromaffin cells through diamond-based MEAs.- Vesicle collision protocols for
the study of quantum size and exocytotic fraction released.- Patch
Amperometry and Intracellular Patch Electrochemistry.- Artificial cells for
dissecting exocytosis.- Isolation and purification of chromaffin granules
from adrenal glands and cultured neuroendocrine cells.- Confocal microscopy
studies of F-actin cytoskeleton distribution and dynamics using fluorescent
LifeAct constructs in bovine adrenal chromaffin cells.- Unveiling the
nanoscale dynamics of the exocytic machinery in chromaffin cells with
single-molecule imaging.- Determinationof catecholamines in a small volume
(25µl) of plasma from conscious mouse tail vein.- Quantification of
chromogranin A and its fragments in biological fluids.- Cytotoxicity models
in chromaffin cells to evaluate neuroprotective compounds.