E-grāmata: Chromaffin Cells: Methods and Protocols

Edited by Ricardo Borges

Formāts: PDF+DRM
Sērija : Methods in Molecular Biology 2565
Izdošanas datums: 07-Oct-2022
Izdevniecība: Springer-Verlag New York Inc.
Valoda: eng
ISBN-13: 9781071626719

Citas grāmatas par šo tēmu:

Formāts - PDF+DRM
Cena: 118,37 €*
* ši ir gala cena, t.i., netiek piemērotas nekādas papildus atlaides
Ielikt grozā
Pievienot vēlmju sarakstam
Šī e-grāmata paredzēta tikai personīgai lietošanai. E-grāmatas nav iespējams atgriezt un nauda par iegādātajām e-grāmatām netiek atmaksāta.

Formāts: PDF+DRM
Sērija : Methods in Molecular Biology 2565
Izdošanas datums: 07-Oct-2022
Izdevniecība: Springer-Verlag New York Inc.
Valoda: eng
ISBN-13: 9781071626719

Citas grāmatas par šo tēmu:

DRM restrictions

Kopēšana (kopēt/ievietot):

nav atļauts
Drukāšana:

nav atļauts
Lietošana:

Digitālo tiesību pārvaldība (Digital Rights Management (DRM))
Izdevējs ir piegādājis šo grāmatu šifrētā veidā, kas nozīmē, ka jums ir jāinstalē bezmaksas programmatūra, lai to atbloķētu un lasītu. Lai lasītu šo e-grāmatu, jums ir jāizveido Adobe ID. Vairāk informācijas šeit. E-grāmatu var lasīt un lejupielādēt līdz 6 ierīcēm (vienam lietotājam ar vienu un to pašu Adobe ID).

Nepieciešamā programmatūra
Lai lasītu šo e-grāmatu mobilajā ierīcē (tālrunī vai planšetdatorā), jums būs jāinstalē šī bezmaksas lietotne: PocketBook Reader (iOS / Android)

Lai lejupielādētu un lasītu šo e-grāmatu datorā vai Mac datorā, jums ir nepieciešamid Adobe Digital Editions (šī ir bezmaksas lietotne, kas īpaši izstrādāta e-grāmatām. Tā nav tas pats, kas Adobe Reader, kas, iespējams, jau ir jūsu datorā.)

Jūs nevarat lasīt šo e-grāmatu, izmantojot Amazon Kindle.

This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Rat pheochromocytoma PC12 cells in culture.- Adrenal grafts in the
central nervous system: Chromaffin and chromaffin progenitor cell
transplantation.- Immunocytochemistry of acutely isolated adrenal medullary
chromaffin cells.- Transmission electron microscopy: a method for studying
the adrenochromaffin cells.- Immunogold for protein location in chromaffin
cells.- Visualization of exo- and endocytosis membrane dynamics with
super-resolution STED microscopy.- Online detection of catecholamine release
from the perfused rat adrenal gland.- On-line recording of perifused
chromaffin cells.- Recording of chromaffin cell electrical activity in situ
in acute adrenal slices.- Calcium imaging and amperometric recording in
cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto
rats, and Spontaneously Hypertensive Rats.- Measurements of calcium in
chromaffin cell organelles using targeted aequorins.- Quantification of
secretory granule exocytosis by TIRF imaging and capacitance
measurements.- Membrane capacitance measurements of stimulus-evoked
exocytosis in adrenal chromaffin cell.- Quantal release analysis of
electrochemically active molecules using single-cell
amperometry.- Methodologies for detecting quantal exocytosis in adrenal
chromaffin cells through diamond-based MEAs.- Vesicle collision protocols for
the study of quantum size and exocytotic fraction released.- Patch
Amperometry and Intracellular Patch Electrochemistry.- Artificial cells for
dissecting exocytosis.- Isolation and purification of chromaffin granules
from adrenal glands and cultured neuroendocrine cells.- Confocal microscopy
studies of F-actin cytoskeleton distribution and dynamics using fluorescent
LifeAct constructs in bovine adrenal chromaffin cells.- Unveiling the
nanoscale dynamics of the exocytic machinery in chromaffin cells with
single-molecule imaging.- Determinationof catecholamines in a small volume
(25µl) of plasma from conscious mouse tail vein.- Quantification of
chromogranin A and its fragments in biological fluids.- Cytotoxicity models
in chromaffin cells to evaluate neuroprotective compounds.

Biežāk uzdotie jautājumi par e-grāmatām