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E-grāmata: CRISPR - Biology and Applications: Biology and Applications [Wiley Online]

  • Formāts: 304 pages
  • Sērija : ASM Books
  • Izdošanas datums: 25-Aug-2022
  • Izdevniecība: American Society for Microbiology
  • ISBN-10: 1683673794
  • ISBN-13: 9781683673798
  • Wiley Online
  • Cena: 142,59 €*
  • * this price gives unlimited concurrent access for unlimited time
  • Formāts: 304 pages
  • Sērija : ASM Books
  • Izdošanas datums: 25-Aug-2022
  • Izdevniecība: American Society for Microbiology
  • ISBN-10: 1683673794
  • ISBN-13: 9781683673798

Since their discovery and subsequent development into laboratory tools, CRISPR-Cas systems have revolutionized the science of gene editing and their possible applications continue to expand, from basic research to potentially groundbreaking medical and commercial uses.

Led by a distinguished team of editors, CRISPR: Biology and Applications explores the subject matter needed to delve into this fascinating area. This comprehensive text presents the diversity of CRISPR-Cas systems, the underlying biology of these systems, and CRISPR-based technologies and applications. Topics covered include:

  • Classification and molecular mechanisms of CRISPR-Cas systems
  • CRISPR-Cas evolution, regulation, expression, and function
  • Uses for gene editing and modulation of gene expression
  • CRISPR-based antimicrobials and phage resistance for medical and industrial purposes

Written by internationally renowned authors, CRISPR: Biology and Applications serves as both an introductory guide for those new to the field and a ready reference for seasoned researchers whose work touches this evolving and headline-making science.

Contributors vii
Preface xi
SECTION I Diversity of CRISPR-Cas Systems
1(120)
1 CRISPR-Cas Systems: Core Features and Common Mechanisms
3(10)
Rodolphe Barrangou
Luciano A. Marraffini
Erik J. Sontheimer
2 Evolutionary Classification of CRISPR-Cas Systems
13(26)
Kira S. Makarova
Yuri I. Wolf
Eugene V. Koonin
3 Molecular Mechanisms of Type I CRISPR-Cas Systems
39(14)
John van der Oost
4 Molecular Mechanisms of Type II CRISPR-Cas Systems
53(18)
Tautvydas Karvelis
Virginijus Siksnys
5 Mechanism of Type III CRISPR-Cas Immunity
71(14)
Luciano A. Marraffini
6 Type V CRISPR-Cas Systems
85(14)
Morgan Quinn Beckett
Anita Ramachandran
Scott Bailey
7 CRISPR-Cas13: Biology, Mechanism, and Applications of RNA-Guided, RNA-Targeting CRISPR Systems
99(22)
Omar O. Abudayyeh
Jonathan S. Gootenberg
SECTION II CRISPR-Cas Biology
121(42)
8 CRISPR-Cas, Horizontal Gene Transfer, and the Flexible (Pan) Genome
123(8)
Uri Gophna
9 Evasion Tactics Manifested by Bacteriophages against Bacterial Immunity
131(14)
Jenny Y. Zhang
Sutharsan Govindarajan
Joseph Bondy-Denomy
10 Regulation of CRISPR-Cas Expression and Function
145(18)
Leah M. Smith
Aroa Rey Campa
Peter C. Fineran
SECTION III CRISPR-Based Technologies and Applications
163(118)
11 Genome Editing with CRISPR-Cas Systems
165(30)
Peter Lotfy
Patrick D. Hsu
12 Genetic and Epigenetic Modulation of Gene Expression by CRISPR-dCas Systems
195(18)
Jasprina N. Noordermeer
Crystal Chen
Lei S. Qi
13 CRISPR Screens
213(20)
Jonathan D. D'Gama
Joseph S. Park
Matthew K. Waldor
14 CRISPR-Based Antimicrobials
233(16)
Justen Russell
David Bikard
15 Exploiting CRISPR-Cas Systems To Provide Phage Resistance in Industrial Bacteria
249(18)
Rodolphe Barrangou
Avery Roberts
16 Recording Biological Information with CRISPR-Cas Systems
267(14)
Mariia Y. Cherepkova
Tanmay Tanna
Randall J. Platt
Afterword 281(2)
Index 283