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E-grāmata: Interleukin Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Medicine 60
  • Izdošanas datums: 02-Feb-2008
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781592591466
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Interleukin ProtocolsPalielināt
  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Medicine 60
  • Izdošanas datums: 02-Feb-2008
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781592591466
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Neuroscientists, psychiatrists, pathologists, oncologists, surgeons, microbiologists, and other researchers and physicians from around the world present 36 contributions which address the major techniques in interleukin research methodologies currently available. The five sections address methods for assaying interleukin protein and mRNA, including the use of ELISA; the assays of interleukins in specific pathologies; the assaying of interleukins in different biological fluids relevant to disease; and newer methods in the study of interleukin signal transduction, analysis of polymorphisms in interleukin genes, and the use of cDNA arrays. Of likely interest to a wide array of scientific investigators including molecular and cell biologists and immunologists. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Luke O'Neill and Andrew Bowie bring together a collection of standard and advanced methods for measuring these powerful agents. Their readily reproducible techniques range from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the study of interleukin signal transduction. Newer techniques are also covered, including the analysis of interleukin gene polymorphisms and the use of cDNA microarrays. Many of the assays are geared to specific pathologies, including breast cancer, depression, psoriasis, Grave's disease, migraine, and myocardial infarction. Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics.

Recenzijas

"this volume offers protocols for measurement of various interleukins. Most are of sufficient detail so as to serve as a laboratory guide for investigators. Investigators with a specific interest in analyzing a given interleukin will find this volume helpful. "-Doody's Health Sciences Book Review Journal





"Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics." - Journal of Investigational Allergology and Clinical immunology





"A variety of techniques is represented in 36 chapters which are written in a concise, uniform format b y authors who obviously have a lot of hands-on experience with the methods covered...If you are working in this area, put this book on a shelf in the lab and it will not be just recently started graduate students who will gracefully turn to it. - Chembiochem





"The protocols that are described in the book are user friendly, in the sense that they are not only well detailed and easy to follow but also reliable. The researchers that are naive in the field will find a lot of useful information in the book whereas the experienced ones will be able to use the book as a source of additional information on the methods already in use. A must therefore for the psychoneuroendocrinologist with a working interest in the relationships between the (neuroendocrine) brain and the immune system." -Psychoneuroendocriniology

Preface v Contributors xi Part I: General Methods for Assaying Interleukin Protein and MRNA 1(174) ELISAs and Interleukin Research 3(14) Catherine Greene ELISPOT Technique for Assaying Interleukins 17(12) Almuth Christine Hauer Mona Bajaj-Elliott Measurement of Interleukins by ELISPOT Assay with Particular Application to Dual-Color Analysis (Stardust Assay) 29(12) Yoshihiro Okamoto Mikio Nishida Assaying Interleukins Intracellulary by FACS 41(8) Liam OMahony Maurice ODonoghue Basic RT-PCR for Measurement of Cytokine Expression 49(10) Mona Bajaj-Elliott Almuth Christine Hauer Quantitative PCR for Measurement of Cytokine Expression 59(18) Ellen M. Nilsen Finn-Eirik Johansen Per Brandtzaeg Single Cell RT-PCR for the Detection of Cytokine mRNA 77(6) Moshe Ligumsky Hovav Nechushtan Ehud Razin Quantitation of Cytokine mRNA by Flash-Type Bioluminescence 83(16) Jeffrey K. Actor Measurement of Interleukin mRNA by Northern Blotting Using a Nonradioactive Detection Method 99(12) Ginette Tardif John A. Di Battista Jean-Pierre Pelletier Johanne Martel-Pelletier Quantification of Mouse IL-6 and TNF-α mRNA Using Xplore Assays 111(14) Scott W. Van Arsdell Kevin P. Murphy Csaba Pazmany Diane Erickson Mark D. Moody Measurement of Cytokine and Chemokine mRNA Using Nonisotopic Multiprobe RNase Protection Assay 125(20) Zheng-Ming Wang Roman Dziarski Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography 145(12) Terry M. Phillips Benjamin F. Dickens Detection of Cytokines by Immunohistochemistry 157(6) Mahmoud Huleihel Whole Blood Assays and the Influence of Cicadian Rhythmicity on Human Cytokine Measurement 163(12) Nikolai Petrovsky Part II: Illustration of Methodologies Using Individual Interleukins 175(30) Assaying Interleukin-8 177(8) Paul N. Moynagh Measurement of Interleukin-2 by Bioassay 185(8) Lyanne Weston Assaying Interleukin-15 193(12) Amos Douvdevani Michal Weiler Eli Lewis Part III: Assaying Interleukins in Particular Pathologies 205(78) Assaying Interleukin-6 in Breast Cancer 207(10) Judith Harmey Amanda Haverty Deirdre Foley David Bouchier-Hayes Cytokines, Stress, and Depressive Illness 217(22) Hymie Anisman Shawn Hayley Arun V. Ravindran Zul Merali Jenna Griffiths Measurement of Interleukins in Cutaneous Disorders: Competitive RT-PCR for Determination of IL-10 mRNA Expression in Psoriasis 239(12) Khusru Asadullah Antje Haeussler-Quade Wolf Dietrich Doecke Wolfram Sterry Hans-Dieter Volk Interleukins in Graves Disease 251(12) Jan Komorowski Evaluation of Cytokines in Migraine Patients by ELISA 263(10) Irene Munno Mariarosaria Marinaro Giuseppe Lacedra Antonia Bassi Vincenzo Centonze Assaying Interleukins in Plasma in the Course of Acute Myocardial Infarction 273(10) Radek Pudil Part IV: Assaying Interleukins in Particular Biological Fluids 283(72) Measurement of Cytokines in Peritoneal Fluids 285(8) Reijo Punnonen Juha Punnonen Measurement of Cytokines in Seminal Plasma 293(12) Marek Glezerman Eitan Lunenfeld Mahmoud Huleihel Measurement of Cytokines in Induced Sputum: Application to the Investigation of Asthma and COPD 305(8) Peter G. Gibson Jodie L. Simpson Cytokines in Blister Fluids 313(14) Franco Ameglio Luciano DAuria Measurement of Cytokines in Synovial Fluid 327(10) Fabrizio De Benedetti Measurement of Cytokines in Cerebrospinal Fluid 337(8) Toshiyuki Yokoyama Detection of Cytokines in Tears 345(10) Archana Thakur Mark D. P. Willcox Part V: Newer Techniques: Interleukin Signal Transduction, Recombinant Interleukin Production, Interleukin Gene Polymorphisms, and the Use of cDNA Arrays 355(84) Measurement of Phosphoinositide 3-Kinase Activation by Interleukins 357(12) Stephen G. Ward Analysis of Interleukin-2 Signaling Using Affinity Precipitations and Polyacrylamide Gel Electrophoresis 369(16) Paul Brennan Veronica Athie-Morales Applications for Green Fluorescent Protein in Cell Signaling 385(8) Paul Brennan Emmanuelle Astoul Use of Pichia pastoris for Production of Recombinant Cytokines 393(16) Kevin P. Murphy Csaba Pazmany Mark D. Moody Analysis of Single Nucleotide Polymorphisms in the Interleukin-1B Gene Using 5 Nuclease Assays 409(10) Emad M. El-Omar Measurement of Cytokine and Chemokine Gene Expression Patterns Using cDNA Array 419(20) Zheng-Ming Wang Roman Dziarski Index 439
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