This volume details techniques on the study of Isolation, characterization, and exploration of actinobacteria in industrial, food, agricultural, and environmental microbiology. Chapters cover a wide range of basic and advanced techniques associated with research on isolation, characterization and identification of actinobacteria in soil, sediment, estuarine, water, Saltpan, Mangroves, plants, lichens, sea weeds, sea grass, animals-crab, snail, shrimp.
Authoritative and cutting-edge, Methods in Actinobacteriology aims to be a useful practical guide to researches to help further their study in this field.
Isolation of Actinobacteria from Soil and Marine Sediment
Samples.- Isolation of Actinobacteria from Water Sources.- Isolation of
Actinobacteria from Deep Sea.- Isolation of Actinobacteria from
Estuary.- Isolation of Actinobacteria from Sponges.- Isolation of
Actinobacteria from Shrimp.- Isolation of Actinobacteria from Coral
Reef.- Isolation of Actinobacteria from Earthworm Cast.- Isolation of
Actinobacteria from Compost Samples.- Isolation of Gut Actinobacteria from
Fecal and Tissue Samples.- Isolation of Gut Actinobacteria from
Termites.- Isolation of Gut Actinobacteria from Fishes.- Isolation of
Actinobacteria from Mangrove Plants.- Isolation of Actinobacteria from
Seaweeds.- Isolation of Actinobacteria from Hills.- Isolation of Endophytic
Actinobacteria from Flowers, Fruits, and Seeds of Higher Plants.- Isolation
of Actinobacteria from Desert Soils.- Isolation of Epiphytic Actinobacteria
from Rhizosphere of Spermatophytes.- Isolation of Epiphytic Actinobacteria
from Lichens.- Isolation of Endophytic Actinobacteria from
Lichens.- Isolation of Psychrophilic and Psychrotolerant
Actinobacteria.- Isolation of Halophilic Actinobacteria from different
habitats.- Isolation of Thermophilic Actinobacteria from Different
Habitats.- Isolation of Stone Dwelling Actinobacteria.- Cultivation
Techniques of Rare Actinobacteria.- Chemo-taxonomical Characterization of
Actinobacteria.- Application of Bioinformatic Tools for Phylogenetic
Analysis.- Methods for Whole-genome Analysis of Actinobacteria through
Bioinformatics Approaches.- Nanopore Based Long Read Sequencing Technology to
Obtain Highly Contiguous Whole Genome Sequence of Actinobacterial Genomes
like Streptomyces sp..- Mining genomes of Actinobacteria.- Comparative
genomics of Actinobacteria.- Biosynthetic Gene Cluster Analysis in
Micromonospora Species Using Antismash: Secondary Metabolite Genome Mining
Pipeline.- Biosynthetic Gene Cluster Analysis in Actinobacterial Genera
Streptomyces spp..- PCR based Determination of Secondary Metabolite Genes in
Actinobacterial Cultures. Molecular Mass Determination of Bacteriocin by
SDS-PAGE analysis.- MALDI-TOF Analysis of Actinobacterial Peptides with
Respect to MASCOT Database.- Protocols for Preclinical Evaluation and
Molecular Docking of Antimicrobial Compounds from Streptomyces sp.- Drug
Likeliness Evaluation, ADME-Toxicity Investigation, Docking modes between the
Ligand and the target Enzyme and Active Site Prediction.- Energy-based
Pharmacophore Hypothesis Combined with Molecular Simulation Protocol for The
Screening Of Bioactive Compounds From the Class of Actinobacteria.- Receptor
cavity-based approach combined with Autodock protocol for the screening
of antiviral compounds from Streptomyces sp..- Pharmacophore Based Hypothesis
Combined with Molecular Docking Protocol for the Screening Of Anti-Cancer
Compounds from Streptomyces sp..- Methods of Identification and Validation of
Drug Target.- Transcriptome profiles of Streptomyces sp..- Culture of
Actinobacteria, Isolation, and Characterization of their Bioactive
Compounds.- Miniaturized Production of Bioactive Extracts from
Actinobacteria.- Screening, Characterization, and Identification of
Antibacterial Compounds From Actinobacteria.- Isolation, Identification, and
Screening of Polyene Antifungal Compound Producing Streptomyces sampsonii
MDCE7 from agroforestry soil.- Screening of Actinobacterial Cultures for
Antimycobacterial Activity using Mycobacterium smegmatis.- Screening of
Actinobacterial Extracts/Compounds for Antimycobacterial Activity by
Luciferase Reporter Phage (LRP) Assay.- Screening of Actinobacteria for
Anti-TB Activity by MicroplateAlamarBlue Assay (MABA).- Screening of
Actinobacteria for anti-TB Activity by Agar Dilution Assay.- Antiplasmodial
activity of Halophilic Actinobacteria.- An invitroAntiamoebic Activity of
Actinobacteria.- Screening for Antiviral Activity: MTT Assay.- Screening for
Anticancer Activity: 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium
(MTT) Assay.- Screening for Anticancer Activity: Dual Staining
Method.- Screening for Anticancer Activity: Neutral Red Uptake
Assay.- Screening for Anticancer Activity: Lactic Acid Dehydrogenase
Assay.- Screening for Anticancer Activity: DNA Fragmentation
Assay.- Screening for Anticancer Activity: Trypan Blue Exclusion Assay.- In
vitro Evaluation of Antimitotic Properties Of Actinobacterial Extracts Using
Onion Root Tip Assay.- Screening for Antioxidant Activity:
Diphenylpicrylhydrazine (DPPH) Assay.- Screening for Antioxidant Activity:
Nitric Oxide Scavenging Assay.- Screening for Antioxidant Activity: Metal
Chelating Assay.- Screening for Antioxidant Activity: Total Antioxidant
Assay.- Screening for Antioxidant Activity: Hydrogen Peroxide Scavenging
Assay.- In vitro Evaluation Of Actinobacterial Extracts For Anti Inflammatory
Properties.- Screening and production of anti freeze Proteins from
Actinobacteria.- In vitro Evaluation of Actinobacterial Extracts for
Immunomodulatory Properties.- Screening of Actinobacteria for Enzyme
Inhibitor Activity.- Screening of Actinobacteria for Quorum Sensing
Inhibition.- Screening of Actinobacterial Extracts for Anti-biofilm
Activity.- Screening of Actinobacteria for Microbial Induced Calcium
Precipitation (MICP).- Immobilization of Actinobacterial Cells Sodium
Alginate and Calcium Chloride Method.- Production of Actinobacteria Amylase
by Fermentation in Solid State Using Residues Of Licuri Palm
(Syagruscoronata).- Production Of Lipase By Actinobacteria.- Isolation And
Cultivation Of Actinobacteria By Submerged Fermentation For The Production Of
Keratinase.- Screening of Cellulase from Actinobacteria.- Screening of
Pectinase from Actinobacteria.- Screening of Protease from
Actinobacteria.- Screening and analysis of Actinobacterial Bioherbicides For
Weed Management.- In vitro Assessment of Actinobacteria for Survivability
Under Simulated Gastro-Intestinal Transit.- In vitro Assessment of
Actinobacteria for Survivability Under Simulated Gastro-Intestinal
Transit.- Screening of Actinobacterial Probiotics by Anti-Pathogenic Activity
Test.- Screening and Analysis of Probiotic Actinobacteria in Poultry
Farming.- Vitamin B12 Producing Actinobacteria as Probiotics for Poultry
Production.- Qualitative and Quantitative Estimation of Phosphate
Solubilizing Actinobacteria.- Estimation of Nitrogen Production by
Actinobacteria.- Screening of Actinobacteria for Siderophore
Production.- Isolation and Screening of Symbiotic Actinobacteria From Root
Nodules of Actinorhizal plant Casuarina sp.- Biocontrol Activity of
Actinobacteria Against Plant Pathogens.- An In vitro nematicidalactivity
ofactinobacteria- Juvenile mortality and Egg hatching inhibition.- Estimation
of Auxin Production by Actinobacteria.- Screening of Actinobacteria for
Mosquitocidal Activity.- Extraction, Characterization, and Identification of
Odorous Metabolites from Streptomyces.- Screening of Actinobacteria for
Biosurfactant Production.- Optimization and Characterization of Biosurfactant
from Streptomyces.- Production of Vitamin B12from Streptomyces
species.- Screening of Microbes for the Production of Pigment
(Melanin).- Exploration and Characterization ofMelanin Pigment Produced by
Actinomycetes.- Isolation and Production of Prodigiosin Pigments from
Streptomyces spp..- Aerobic and Anaerobic Decolourization of Textile Dyes
Using Actinobacteria.- Bioleaching of heavy metals from e-waste using
actinobacteria.- Biosynthesis and Characterization of Silver Nanoparticles
From Actinobacteria.- Biosynthesis and Characterization of Gold Nanoparticles
from Actinobacteria.- Antimicrobial Activity of Extracellular
Green-Synthesized Nanoparticles By Actinobacteria.- Antibiofilm Activity of
Extracellular Green-Synthesized Nanoparticles By Actinobacteria.- Cytotoxic
Activity of Extracellular Green Synthesized Nanoparticles by
Actinobacteria.- Sporicidal Activity of Extracellular Green-Synthesized
Nanoparticles by Actinobacteria.
