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E-grāmata: Phytochemistry of Tinospora cordifolia

(Central Drug Research, India), ,
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Phytochemistry of Tinospora cordifoliaPalielināt
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Tinospora cordifolia stem is used as a tonic, vitalizer, and as a remedy for metabolic disorders to treat allergies, diabetes, dysentery, jaundice, heart diseases, leprosy, rheumatoid arthritis, skin diseases, and urinary disorders. It shows anti-inflammatory, analgesic, antipyretic actions and immunosuppressive effects. This book focuses on providing gender and geographical location-based differences in the phytoconstituents of T. cordifolia by the liquid chromatography mass spectrometric method. These methods have potential use in the quality control of T. cordifolia and the screening of herbal preparations.

Features:











Compilation of ayurvedic features of one of the most important plants of the Indian system of medicines.





Useful for all ayurvedic practitioners, researchers, faculty, students, and herbal product manufacturers.





Application of advance hyphenated LC-MS techniques for variation study in phytoconstituents.
List of Figures
vii
List of Tables
ix
List of Schemes
xi
Preface xiii
Acknowledgments xv
Authors xvii
List of Abbreviations and Units
xix
1 Introduction: Tinospora cordifolia (Amrita)--The Wonder Plant
1(12)
1.1 Natural Habitat and Distribution
2(2)
1.2 Microscopy of Tinospora cordifolia Stem
4(1)
1.3 Ayurvedic Properties
4(2)
1.4 Gross Pharmacological Properties
6(1)
1.5 Phytochemistry
7(6)
2 Phytochemical Analysis and Metabolite Diversity in Tinospora cordifolia
13(50)
2.1 Description of Plant Material
14(1)
2.2 Reagent and Chemicals
15(1)
2.3 Preparation of Extracts
15(2)
2.4 Preparation of Standard and Stock Solutions
17(1)
2.5 DART-TOF-MS Analysis of Intact Stem Samples
17(1)
2.6 DART-TOF-MS Parameters
18(1)
2.7 Chemometric Analysis to Study Discrimination
18(1)
2.8 LC-MS Data Processing for Discrimination
19(1)
2.9 DART-TOF-MS Data Processing for Discrimination
20(1)
2.10 Validation Studies for Chemometric Methods
20(1)
2.11 Optimization of DART-TOF-MS Parameters
21(1)
2.12 DART-TOF-MS Analysis of Male, Female and Geographical Samples
21(4)
2.13 Principal Component Analysis Using DART-TOF-MS Fingerprints
25(1)
2.14 HPLC-ESI-QTOF-MS/MS Conditions
26(1)
2.15 Screening of Phytochemicals by HPLC-ESI-QTOF-MS/MS Analysis
27(1)
2.16 Guanidino Compounds
27(1)
2.17 Protoberberine Alkaloids
28(20)
2.18 Benzylisoquinoline Alkaloids
48(1)
2.19 Aporphine Alkaloids
49(1)
2.20 Other N-Containing Compounds
50(1)
2.21 Ecdysterone and Its Derivatives
50(1)
2.22 Sesquiterpenes
51(1)
2.23 Phytochemical Variation Study in Male and Female Samples
52(3)
2.24 Discrimination in Geographical Samples
55(3)
2.25 Discrimination in Seasonal Samples
58(1)
2.26 Quantitative Estimation
59(1)
2.27 UHPLC Conditions for Quantitation of Alkaloids
60(1)
2.28 ESI-QqQLIT-MS/MS Conditions for Quantitation of Alkaloids
60(3)
3 Correlative Pharmacological Activity of Tinospora cordifolia
63(12)
3.1 Evaluation of Immune Response
65(1)
3.2 Reactive Oxygen Species
65(1)
3.3 CD4/CD8 T and CD19B Lymphocyte Population
66(1)
3.4 Intracellular Cytokines
66(1)
3.5 In Vivo Evaluation of Immune Response
67(3)
3.6 Evaluation of Cell Cytotoxicity
70(3)
3.7 Morphological Assay and Evaluation
73(2)
4 Conclusion
75(2)
References 77(8)
Index 85
Brijesh Kumar is a Professor (AcSIR) and Chief Scientist of sophisticated analytical instrument facility division, CSIR-Central Drug Research Institute, Lucknow, India. Currently he is facility in charge at Sophisticated Analytical Instrument Facility (SAIF) division of CSIR-CDRI. He has completed his PhD from CSIR-CDRI Lucknow (Dr. R.M.L Avadh University Faizabad UP, India). He has to his credit 7 book chapters, one book and 145 papers in International journal of repute. His current area of research is applications of Mass Spectrometry (DART MS/Q-TOF LC-MS/4000 Q Trap LC-MS/ Orbitrap MSn) for qualitative and quantitative analysis of molecules for quality control and authentication/standardization of Indian medicinal plants/parts and their herbal formulations. He is also involved in identification of marker compounds using statistical software to check adulteration/substitution.

Vikas Bajpai completed his PhD from the Academy of Scientific and Innovative Research (AcSIR), New Delhi, India and carried out his research work under supervision of Dr. Brijesh Kumar at CSIR-Central Drug research Institute Lucknow. His research interest includes development and validation of LC-MS/MS methods for qualitative and quantitative analysis of phytochemicals in Indian medicinal plants.

Nikhil Kumar - a plant physiologist by training and worked in different capacities for about thirty years and finally superannuated from CSIR-National Botanical Research Institute (NBRI), Lucknow, India. He has worked on one of the very important cultural plants of India including South East Asia. Piper betle which effectively broadened his understanding as how plants contributed in the development of human skills. He has published more than fifty research papers in national and international journals. He brought to focus the ascpect of dioecy in Piper betle and Tinospora cordifolia and its possible functional implications in adaptation and biological activities.
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