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E-grāmata: Phytochemistry of Tinospora cordifolia [Taylor & Francis e-book]

, , (Central Drug Research, India)
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Phytochemistry of Tinospora cordifoliaPalielināt
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Tinospora cordifolia stem is used as a tonic, vitalizer, and as a remedy for metabolic disorders to treat allergies, diabetes, dysentery, jaundice, heart diseases, leprosy, rheumatoid arthritis, skin diseases, and urinary disorders. It shows anti-inflammatory, analgesic, antipyretic actions and immunosuppressive effects. This book focuses on providing gender and geographical location-based differences in the phytoconstituents of T. cordifolia by the liquid chromatography mass spectrometric method. These methods have potential use in the quality control of T. cordifolia and the screening of herbal preparations.

Features:

  • Compilation of ayurvedic features of one of the most important plants of the Indian system of medicines.

  • Useful for all ayurvedic practitioners, researchers, faculty, students, and herbal product manufacturers.

    • Application of advance hyphenated LC-MS techniques for variation study in phytoconstituents.

    • List of Figures
    		vii
    List of Tables
    		ix
    List of Schemes
    		xi
    Preface xiii
    Acknowledgments xv
    Authors xvii
    List of Abbreviations and Units
    		xix
    1 Introduction: Tinospora cordifolia (Amrita)---The Wonder Plant
    		1(12)
    1.1 Natural Habitat and Distribution
    		2(2)
    1.2 Microscopy of Tinospora cordifolia Stem
    		4(1)
    1.3 Ayurvedic Properties
    		4(2)
    1.4 Gross Pharmacological Properties
    		6(1)
    1.5 Phytochemistry
    		7(6)
    2 Phytochemical Analysis and Metabolite Diversity in Tinospora cordifolia
    		13(50)
    2.1 Description of Plant Material
    		14(1)
    2.2 Reagent and Chemicals
    		15(1)
    2.3 Preparation of Extracts
    		15(2)
    2.4 Preparation of Standard and Stock Solutions
    		17(1)
    2.5 DART-TOF-MS Analysis of Intact Stem Samples
    		17(1)
    2.6 DART-TOF-MS Parameters
    		18(1)
    2.7 Chemometric Analysis to Study Discrimination
    		18(1)
    2.8 LC-MS Data Processing for Discrimination
    		19(1)
    2.9 DART-TOF-MS Data Processing for Discrimination
    		20(1)
    2.10 Validation Studies for Chemometric Methods
    		20(1)
    2.11 Optimization of DART-TOF-MS Parameters
    		21(1)
    2.12 DART-TOF-MS Analysis of Male, Female and Geographical Samples
    		21(4)
    2.13 Principal Component Analysis Using DART-TOF-MS Fingerprints
    		25(1)
    2.14 HPLC-ESI-QTOF-MS/MS Conditions
    		26(1)
    2.15 Screening of Phytochemicals by HPLC-ESI-QTOF-MS/MS Analysis
    		27(1)
    2.16 Guanidino Compounds
    		27(1)
    2.17 Protoberberine Alkaloids
    		28(20)
    2.18 Benzylisoquinoline Alkaloids
    		48(1)
    2.19 Aporphine Alkaloids
    		49(1)
    2.20 Other N-Containing Compounds
    		50(1)
    2.21 Ecdysterone and Its Derivatives
    		50(1)
    2.22 Sesquiterpenes
    		51(1)
    2.23 Phytochemical Variation Study in Male and Female Samples
    		52(3)
    2.24 Discrimination in Geographical Samples
    		55(3)
    2.25 Discrimination in Seasonal Samples
    		58(1)
    2.26 Quantitative Estimation
    		59(1)
    2.27 UHPLC Conditions for Quantitation of Alkaloids
    		60(1)
    2.28 ESI-QqQLIT-MS/MS Conditions for Quantitation of Alkaloids
    		60(3)
    3 Correlative Pharmacological Activity of Tinospora cordifolia
    		63(12)
    3.1 Evaluation of Immune Response
    		65(1)
    3.2 Reactive Oxygen Species
    		65(1)
    3.3 CD4/CD8 T and CD19 B Lymphocyte Population
    		66(1)
    3.4 Intracellular Cytokines
    		66(1)
    3.5 In Vivo Evaluation of Immune Response
    		67(3)
    3.6 Evaluation of Cell Cytotoxicity
    		70(3)
    3.7 Morphological Assay and Evaluation
    		73(2)
    4 Conclusion
    		75(2)
    References 77(8)
    Index 85
    Brijesh Kumar is a Professor (AcSIR) and Chief Scientist of sophisticated analytical instrument facility division, CSIR-Central Drug Research Institute, Lucknow, India. Currently he is facility in charge at Sophisticated Analytical Instrument Facility (SAIF) division of CSIR-CDRI. He has completed his PhD from CSIR-CDRI Lucknow (Dr. R.M.L Avadh University Faizabad UP, India). He has to his credit 7 book chapters, one book and 145 papers in International journal of repute. His current area of research is applications of Mass Spectrometry (DART MS/Q-TOF LC-MS/4000 Q Trap LC-MS/ Orbitrap MSn) for qualitative and quantitative analysis of molecules for quality control and authentication/standardization of Indian medicinal plants/parts and their herbal formulations. He is also involved in identification of marker compounds using statistical software to check adulteration/substitution.

    Vikas Bajpai completed his PhD from the Academy of Scientific and Innovative Research (AcSIR), New Delhi, India and carried out his research work under supervision of Dr. Brijesh Kumar at CSIR-Central Drug research Institute Lucknow. His research interest includes development and validation of LC-MS/MS methods for qualitative and quantitative analysis of phytochemicals in Indian medicinal plants.

    Nikhil Kumar - a plant physiologist by training and worked in different capacities for about thirty years and finally superannuated from CSIR-National Botanical Research Institute (NBRI), Lucknow, India. He has worked on one of the very important cultural plants of India including South East Asia. Piper betle which effectively broadened his understanding as how plants contributed in the development of human skills. He has published more than fifty research papers in national and international journals. He brought to focus the ascpect of dioecy in Piper betle and Tinospora cordifolia and its possible functional implications in adaptation and biological activities.
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