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Plant Genome Editing with CRISPR Systems: Methods and Protocols 1st ed. 2019 [Hardback]

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  • Formāts: Hardback, 367 pages, height x width: 254x178 mm, weight: 943 g, 65 Illustrations, color; 8 Illustrations, black and white; XV, 367 p. 73 illus., 65 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 1917
  • Izdošanas datums: 05-Jan-2019
  • Izdevniecība: Humana Press Inc.
  • ISBN-10: 1493989901
  • ISBN-13: 9781493989904
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Plant Genome Editing with CRISPR Systems: Methods and Protocols 1st ed. 2019Palielināt
  • Formāts: Hardback, 367 pages, height x width: 254x178 mm, weight: 943 g, 65 Illustrations, color; 8 Illustrations, black and white; XV, 367 p. 73 illus., 65 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 1917
  • Izdošanas datums: 05-Jan-2019
  • Izdevniecība: Humana Press Inc.
  • ISBN-10: 1493989901
  • ISBN-13: 9781493989904
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781493989904.html
Keywords:

This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

Plant DNA Repair Pathways and their Applications in Genome
Editing.- Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR
Method.- Decoding Sanger-Sequencing Chromatograms from CRISPR-Induced
Mutations.- Creating Large Chromosomal Deletions in Rice using
CRISPR/Cas9.- A Multiplexed CRISPR-Cas9 Editing System Based on the
Endogenous tRNA Processing.- A Single Transcript CRISPR-Cas9 System for
Multiplex Genome Editing in Plants.- CRISPR-Act2.0: An Improved Multiplexed
System for Plant Transcriptional Activation.- Generating
Photoperiod-Sensitive Genic Male Sterile Rice Lines with
CRISPR-Cas9.- Knocking Out MicroRNA Genes in Rice with CRISPR/Cas9.- An
Agrobacterium-Mediated CRISPR-Cas9 Platform for Genome Editing in
Maize.- Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited
Arabidopsis Plants.- Creating Targeted Gene Knock-Outs in Brassica oleracea
using CRISPR/Cas9.- Application of CRISPR/Cas9-Mediated Gene Editing in
Tomato.- Genome Editing in Potato with CRISPR/Cas9.- Visual Assay for Gene
Editing using a CRISPR/Cas9 System in Carrot Cells.- Genome Editing in
Soybean with CRISPR/Cas9.- Genome Editing in Citrus Tree with
CRISPR/Cas9.- Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a)
Systems.- Editing a Stomatal Developmental Gene in Rice with
CRISPR/Cpf1.- Targeted Mutagenesis using FnCpf1 in Tobacco.- Gene Replacement
by Intron Targeting with CRISPR-Cas9.- Targeted Base Editing with
CRISPR-Deaminase in Tomato.- Virus-Mediated Genome Editing in Plants using
the CRISPR/Cas9 System.- Biolistic Delivery of CRISPR/Cas9 with
Ribonucleoprotein Complex in Wheat.- DNA-Free Genome Editing via
Ribonucleoprotein (RNP) Delivery of CRISPR/Cas in Lettuce.- An Automated
Protoplast Transformation System.