E-grāmata: Plant Genome Editing with CRISPR Systems: Methods and Protocols

Plant DNA Repair Pathways and their Applications in Genome
Editing.- Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR
Method.- Decoding Sanger-Sequencing Chromatograms from CRISPR-Induced
Mutations.- Creating Large Chromosomal Deletions in Rice using
CRISPR/Cas9.- A Multiplexed CRISPR-Cas9 Editing System Based on the
Endogenous tRNA Processing.- A Single Transcript CRISPR-Cas9 System for
Multiplex Genome Editing in Plants.- CRISPR-Act2.0: An Improved Multiplexed
System for Plant Transcriptional Activation.- Generating
Photoperiod-Sensitive Genic Male Sterile Rice Lines with
CRISPR-Cas9.- Knocking Out MicroRNA Genes in Rice with CRISPR/Cas9.- An
Agrobacterium-Mediated CRISPR-Cas9 Platform for Genome Editing in
Maize.- Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited
Arabidopsis Plants.- Creating Targeted Gene Knock-Outs in Brassica oleracea
using CRISPR/Cas9.- Application of CRISPR/Cas9-Mediated Gene Editing in
Tomato.- Genome Editing in Potato with CRISPR/Cas9.- Visual Assay for Gene
Editing using a CRISPR/Cas9 System in Carrot Cells.- Genome Editing in
Soybean with CRISPR/Cas9.- Genome Editing in Citrus Tree with
CRISPR/Cas9.- Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a)
Systems.- Editing a Stomatal Developmental Gene in Rice with
CRISPR/Cpf1.- Targeted Mutagenesis using FnCpf1 in Tobacco.- Gene Replacement
by Intron Targeting with CRISPR-Cas9.- Targeted Base Editing with
CRISPR-Deaminase in Tomato.- Virus-Mediated Genome Editing in Plants using
the CRISPR/Cas9 System.- Biolistic Delivery of CRISPR/Cas9 with
Ribonucleoprotein Complex in Wheat.- DNA-Free Genome Editing via
Ribonucleoprotein (RNP) Delivery of CRISPR/Cas in Lettuce.- An Automated
Protoplast Transformation System.