E-grāmata: Rheumatoid Arthritis: Methods and Protocols

Collagen-induced arthritis models.- Human xenograft model.- Scaffolded
chondrogenic spheroid-engrafted model.- Denervation-induced sarcopenia
model.- Long-term constant subcutaneous drug administration.- Clinical
Scoring of Disease Activity in Animal Models.- Histological analyses of
arthritic joints in collagen-induced arthritis model mice.- Preparation of
Joint Extracts.- Production of immunizing antigen proteoliposome using
cell-free protein synthesis system.- Re-construction of protein/liposome
complex.- Production of neutralizing antibody.- Autoantibody profiling using
human autoantigen protein array and AlphaScreen.- Generation of Specific
Aptamers.- Detailed Protocol for Predicting 3D Structure of DNA Aptamers and
Performing In Silico Docking Calculations.- RNA interference ex
vivo.- Lentiviral-mediated systemic RNA interference in vivo.- Lentiviral
production platform.- Mesenchymal stem cell engineering.- Screening of Ca2+
influx in lymphocytes.- Single-cell Ca2+ imaging.- Electrophysiological
methods to measure Ca2+ current.- Evaluation of mitochondrial respiratory
function in murine splenocytes.- The Functional Assessment of T
Cells.- Release of antibodies and cytokines from B cells.- Evaluation of
autoreactive responses.- Macrophage polarization and osteoclast
differentiation.- Scanning electron microscopic analysis of the
bone-resorption activity in mature osteoclasts.- Animal Models of
Vasculitis.- Evaluation of skin damage under UV exposure.- Bulk RNA-seq
assessment of murine spleen  using a portable MinION sequencing
device.- Institutional review board considerations for clinical
trials.- Design an intervention study.- Assessment of disease activity,
structural damage, and function in rheumatoid arthritis.- Assessment of
musculoskeletal ultrasound of rheumatoid arthritis.- 16S rRNA gene amplicon
analysis of human gut microbiota.