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Rheumatoid Arthritis: Methods and Protocols Second Edition 2024 [Hardback]

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  • Formāts: Hardback, 354 pages, height x width: 254x178 mm, weight: 889 g, 52 Illustrations, color; 31 Illustrations, black and white; XI, 354 p. 83 illus., 52 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2766
  • Izdošanas datums: 26-Jan-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071636812
  • ISBN-13: 9781071636817
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  • Formāts: Hardback, 354 pages, height x width: 254x178 mm, weight: 889 g, 52 Illustrations, color; 31 Illustrations, black and white; XI, 354 p. 83 illus., 52 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2766
  • Izdošanas datums: 26-Jan-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071636812
  • ISBN-13: 9781071636817
Citas grāmatas par šo tēmu:
This second edition explores standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.





 





Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols,  Second Edition aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study. 
Collagen-induced arthritis models.- Human xenograft model.- Scaffolded
chondrogenic spheroid-engrafted model.- Denervation-induced sarcopenia
model.- Long-term constant subcutaneous drug administration.- Clinical
Scoring of Disease Activity in Animal Models.- Histological analyses of
arthritic joints in collagen-induced arthritis model mice.- Preparation of
Joint Extracts.- Production of immunizing antigen proteoliposome using
cell-free protein synthesis system.- Re-construction of protein/liposome
complex.- Production of neutralizing antibody.- Autoantibody profiling using
human autoantigen protein array and AlphaScreen.- Generation of Specific
Aptamers.- Detailed Protocol for Predicting 3D Structure of DNA Aptamers and
Performing In Silico Docking Calculations.- RNA interference ex
vivo.- Lentiviral-mediated systemic RNA interference in vivo.- Lentiviral
production platform.- Mesenchymal stem cell engineering.- Screening of Ca2+
influx in lymphocytes.- Single-cell Ca2+ imaging.- Electrophysiological
methods to measure Ca2+ current.- Evaluation of mitochondrial respiratory
function in murine splenocytes.- The Functional Assessment of T
Cells.- Release of antibodies and cytokines from B cells.- Evaluation of
autoreactive responses.- Macrophage polarization and osteoclast
differentiation.- Scanning electron microscopic analysis of the
bone-resorption activity in mature osteoclasts.- Animal Models of
Vasculitis.- Evaluation of skin damage under UV exposure.- Bulk RNA-seq
assessment of murine spleen  using a portable MinION sequencing
device.- Institutional review board considerations for clinical
trials.- Design an intervention study.- Assessment of disease activity,
structural damage, and function in rheumatoid arthritis.- Assessment of
musculoskeletal ultrasound of rheumatoid arthritis.- 16S rRNA gene amplicon
analysis of human gut microbiota.